Clinical Nutrition Center, Shanghai XinHua Hospital, Shanghai, China.
Int J Vitam Nutr Res. 2009 Jul;79(4):255-63. doi: 10.1024/0300-9831.79.4.255.
To evaluate the effect of vitamin E on the level of oxidative stress in diet-induced obese Sprague-Dawley rats.
Thirty weaning male rats were placed into three groups with 10 animals each: a control group with normal chow, a diet-induced obesity group (DIO) with high-fat diet, and an intervention group with high-fat diet supplemented with vitamin E (VE, 350 mg/kg). Blood and adipose tissue were collected from rats after 10 weeks. Biomarkers of oxidative stress were detected for plasma 8-epi-prostaglandin- F(2)alpha (8-epi-PGF(2)alpha), thiobarbituric acid-reactive substances (TBARS), total anti-oxidative capacity (TAOC), alpha-tocopherol, superoxide dismutase (SOD) activity, and glutathione peroxidase activity (GPx). Lipid and glucose metabolism parameters such as plasma glucose, insulin, and triacylglycerol (TG) were also analyzed.
After 10 weeks, all obese rats (both the DIO and VE groups) had higher plasma 8-epi-PGF(2alpha) and TBARS levels than the controls. Their plasma-adjusted alpha-tocopherol, SOD, and GPx activities were lower than the control levels but insulin was higher (p<0.01). The VE intervention increased plasma SOD, GPx, and T-AOC, and decreased 8-epi-PGF(2alpha) (p<0.05). VE intervention also decreased plasma glucose, insulin, and TG levels (p<0.05).
Increased oxidative stress could be an important target for the prevention of obesity-related diseases. Vitamin E has moderate effects for improvement of oxidative stress status and glucose metabolism in the animal model of diet-induced obesity.
评估维生素 E 对饮食诱导肥胖 Sprague-Dawley 大鼠氧化应激水平的影响。
将 30 只断奶雄性大鼠分为 3 组，每组 10 只：正常饲料对照组、高脂肪饮食诱导肥胖组（DIO 组）和高脂肪饮食补充维生素 E 组（VE 组，350mg/kg）。10 周后采集大鼠血液和脂肪组织。检测血浆 8-epi-前列腺素 F(2)alpha（8-epi-PGF(2)alpha）、硫代巴比妥酸反应物质（TBARS）、总抗氧化能力（TAOC）、α-生育酚、超氧化物歧化酶（SOD）活性和谷胱甘肽过氧化物酶活性（GPx）等氧化应激生物标志物。还分析了血糖、胰岛素和三酰甘油（TG）等脂质和葡萄糖代谢参数。
10 周后，所有肥胖大鼠（DIO 组和 VE 组）的血浆 8-epi-PGF(2alpha)和 TBARS 水平均高于对照组。与对照组相比，其血浆调整后的 α-生育酚、SOD 和 GPx 活性降低，但胰岛素升高（p<0.01）。VE 干预增加了血浆 SOD、GPx 和 T-AOC，降低了 8-epi-PGF(2alpha)（p<0.05）。VE 干预还降低了血浆葡萄糖、胰岛素和 TG 水平（p<0.05）。
氧化应激增加可能是预防肥胖相关疾病的一个重要靶点。维生素 E 对饮食诱导肥胖动物模型的氧化应激状态和葡萄糖代谢有一定的改善作用。
