色氨酸-铕(III)配合物与鲱鱼精 DNA 相互作用的光谱研究。
Spectroscopic studies on the interaction between tryptophan-erbium(III) complex and herring sperm DNA.
机构信息
Department of Chemistry, Material Science and Engineering College, Southwest University of Science and Technology, No. 59, Qinglong Road, Mianyang 621010, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2010 May;75(5):1435-42. doi: 10.1016/j.saa.2010.01.013. Epub 2010 Feb 6.
By means of UV and fluorescence spectra, the binding ratios between Er(III)-Trp and DNA in physiological pH environment (pH 7.40) were determined as n(Trp):n(Er(III))=3:1 and (n)ER(III)(Trp)(3):(n)(DNA) = 2:1, and the apparent molar absorptivity of epsilon(Er(III)-Trp-DNA) is 4.33 x 10(5) L mol(-1)cm(-1) which was confirmed by molar ratio method. The binding constants at different temperatures K(B25 degrees C)(theta)=1.93 x 10(4)L mol(-1) and K(B37 degrees C)(theta)=5.28 x 10(3)L mol(-1) were obtained by double reciprocal method. Thermodynamic function computation demonstrates that Delta(r)H(m)(theta) is the primary driving power of the interaction between Er(III)(Trp)(3) and DNA. By combination analysis of the Scatchard method and CD spectrometry, we suggested that the interaction mode between Er(III)(Trp)(3) complex and herring sperm DNA is groove and intercalation bindings.
通过紫外和荧光光谱，在生理 pH 环境（pH 7.40）下测定了 Er(III)-Trp 与 DNA 的结合比，为 n(Trp):n(Er(III))=3:1 和 (n)ER(III)(Trp)(3):(n)(DNA) = 2:1，并且 ε(Er(III)-Trp-DNA)的表观摩尔吸光率通过摩尔比法确认为 4.33 x 10(5) L mol(-1)cm(-1)。通过双倒数法得到不同温度下的结合常数 K(B25 摄氏度)(theta)=1.93 x 10(4)L mol(-1)和 K(B37 摄氏度)(theta)=5.28 x 10(3)L mol(-1)。热力学函数计算表明，Δ(r)H(m)(theta)是 Er(III)(Trp)(3)与 DNA 相互作用的主要驱动力。通过 Scatchard 法和 CD 光谱的综合分析，我们提出 Er(III)(Trp)(3)配合物与鲱鱼精子 DNA 的相互作用模式为沟槽和插入结合。
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