Facultad de Ciencias Bioquímicas, Universidad Nacional deRosario, Rosario, Argentina.
Facultad de Ciencias Médicas, Universidad Nacional de Rosario, Rosario, Argentina.
Diabetol Metab Syndr. 2010 Mar 17;2:15. doi: 10.1186/1758-5996-2-15.
eSS is a rat model of type 2 diabetes characterized by fasting hyperglycemia, glucose intolerance, hyperinsulinemia and early hypertriglyceridemia. Diabetic symptoms worsen during the second year of life as insulin release decreases. In 12-month-old males a diffuse hepatic steatosis was detected. We report the disturbances of lipid metabolism of the model with regard to the diabetic syndrome.
The study was conducted in eight 12-month-old eSS male rats and seven age/weight matched eumetabolic Wistar rats fed with a complete commercial diet al libitum. Fasting plasmatic glucose, insulin, triglycerides, total cholesterol, low-density and high-density lipoprotein, and nonesterified fatty acids levels were measured. Very low density and intermediate-density lipoproteins were analyzed and hepatic lipase activity was determined.
eSS rats developed hyperglycemia and hyperinsulinemia, indicating insulin resistance. Compared with controls, diabetic rats exhibited high plasmatic levels of NEFA, triglycerides (TG), total cholesterol (Chol) and LDL-Chol while high-density lipoprotein (HDL) cholesterol values were reduced. eSS rats also displayed TG-rich VLDL and IDL particles without changes in hepatic lipase activity.
The nonobese eSS rats develop a syndrome characterized by glucose and lipid disorders and hepatic steatosis that may provide new opportunities for studying the pathogenesis of human type 2 diabetes.
eSS 是一种 2 型糖尿病大鼠模型,其特征为空腹高血糖、葡萄糖不耐受、高胰岛素血症和早期高甘油三酯血症。在生命的第二年,随着胰岛素分泌减少,糖尿病症状恶化。在 12 个月大的雄性大鼠中,检测到弥漫性肝脂肪变性。我们报告了该模型在糖尿病综合征方面的脂质代谢紊乱。
本研究在 8 只 12 个月大的 eSS 雄性大鼠和 7 只年龄/体重匹配的代谢正常的 Wistar 大鼠中进行,这些大鼠自由摄入完整的商业饮食。测量空腹血浆葡萄糖、胰岛素、甘油三酯、总胆固醇、低密度脂蛋白和高密度脂蛋白以及非酯化脂肪酸水平。分析极低密度脂蛋白和中间密度脂蛋白,并测定肝脂肪酶活性。
eSS 大鼠出现高血糖和高胰岛素血症,表明胰岛素抵抗。与对照组相比,糖尿病大鼠的血浆 NEFA、甘油三酯 (TG)、总胆固醇 (Chol) 和 LDL-Chol 水平升高,而高密度脂蛋白 (HDL) 胆固醇水平降低。eSS 大鼠还表现出富含 TG 的 VLDL 和 IDL 颗粒,而肝脂肪酶活性没有变化。
非肥胖 eSS 大鼠出现葡萄糖和脂质紊乱以及肝脂肪变性的综合征,这可能为研究人类 2 型糖尿病的发病机制提供新的机会。