在不同 T80 浓度的细胞培养液中 MWCNTs 的分散状态和毒性。
Dispersion state and toxicity of mwCNTs in cell culture medium with different T80 concentrations.
机构信息
Department of Aerospace Engineering, Old Dominion University, Norfolk, VA 23529, USA.
出版信息
Colloids Surf B Biointerfaces. 2010 Jun 15;78(1):36-43. doi: 10.1016/j.colsurfb.2010.02.005. Epub 2010 Feb 19.
In this study, size distribution, zeta potential, shape, and toxicity of multi-walled carbon nanotubes (mwCNTs), and effect of non-ionic detergent Tween 80 (T80) concentrations (0%, 0.2%, and 1%) on the dispersion quality and cell viability are investigated. Nanotubes are suspended in biological solutions (DMEM, RPMI) with three different concentrations (10, 50, and 100 microg/ml) and toxicological investigations are carried on human T-cell leukemia (Jurkat) and human pancreatic carcinoma (PANC1) cell lines. According to light and transmission electron microscopy results, mwCNTs form well-defined and regular bundles in the presence of 1% T80 surfactant; whereas more irregular structures are present in absence of T80. Dispersion quality is represented in terms of the size distribution from dynamic light scattering (DLS) experiments and its second moment. Dispersion quality of the mwCNTs decreases with decreasing T80 concentration, while the constituents of RPMI and DMEM increase the dispersion quality. No significant differences between the dispersive effects of RPMI and DMEM suspensions are observed. Zeta potential of the mwCNTs is measured using electrophoretic light scattering. Variations in the nanotube and T80 concentrations do not change the zeta potential significantly. T80 concentrations above 0.2% are found to be toxic for Jurkat andPANC1 cells.
在这项研究中，研究了多壁碳纳米管（MWCNTs）的粒径分布、Zeta 电位、形态和毒性，以及非离子表面活性剂吐温 80（T80）浓度（0%、0.2%和 1%）对分散质量和细胞活力的影响。将纳米管悬浮在三种不同浓度（10、50 和 100μg/ml）的生物溶液（DMEM、RPMI）中，并在人 T 细胞白血病（Jurkat）和人胰腺癌细胞系（PANC1）上进行毒理学研究。根据光镜和透射电子显微镜的结果，在 1%的 T80 表面活性剂存在下，MWCNTs 形成了定义明确且规则的束；而在没有 T80 的情况下，则存在更不规则的结构。分散质量用动态光散射（DLS）实验及其第二矩表示。随着 T80 浓度的降低，MWCNTs 的分散质量下降，而 RPMI 和 DMEM 的成分则增加了分散质量。未观察到 RPMI 和 DMEM 悬浮液的分散效果有显著差异。MWCNTs 的 Zeta 电位使用电泳光散射测量。纳米管和 T80 浓度的变化对 Zeta 电位没有显著影响。发现 T80 浓度高于 0.2%对 Jurkat 和 PANC1 细胞有毒。
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