Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Mercer University, 3001 Mercer University Drive, Atlanta, Georgia 30341, USA.
Pharm Res. 2011 Jan;28(1):82-94. doi: 10.1007/s11095-010-0122-x. Epub 2010 Mar 31.
To characterize the microchannels created in hairless rat skin by microneedles and investigate their closure following exposure to different occlusive conditions.
Maltose microneedles were characterized by scanning electron microscopy. The microchannels created and their closure when exposed to different conditions was investigated using a variety of techniques.
Microscopic imaging indicates a pyramidal geometry of maltose microneedles with an average length of 559 ± 14 μm and tip radius of 4 μm. Upon insertion into skin, they created microchannels with an average surface diameter of 60 μm and an average depth of 160 ± 20 μm as observed by histological sectioning and confocal microscopy. Skin recovers its barrier function within 3-4 hrs, and microchannels closed within 15 hrs of poration when exposed to environment. However, when occluded, the microchannels remained open for up to 72 hrs in vivo, as observed by calcein imaging, transepidermal water loss measurements and methylene blue staining.
Maltose microneedles penetrated the stratum corneum barrier and created microchannels in skin which completely close within 15 hrs after poration. However, under occluded conditions, barrier recovery can be delayed for up to 72 hrs in vivo.
描述微针在无毛大鼠皮肤中所产生的微通道，并研究在接触不同封闭条件下微通道的闭合情况。
通过扫描电子显微镜对麦芽糖微针进行了表征。使用多种技术研究了在不同条件下微通道的形成及其闭合情况。
显微镜成像显示，麦芽糖微针具有金字塔形的几何形状，平均长度为 559±14μm，尖端半径为 4μm。当插入皮肤时，通过组织学切片和共聚焦显微镜观察到，它们形成了平均表面直径为 60μm、平均深度为 160±20μm 的微通道。皮肤屏障功能在 3-4 小时内恢复，当微通道穿孔后暴露于环境中，15 小时内即可闭合。然而，当被封闭时，通过钙黄绿素成像、经皮水分丢失测量和亚甲蓝染色观察到，微通道在体内可保持开放长达 72 小时。
麦芽糖微针穿透角质层屏障，在皮肤中形成微通道，在穿孔后 15 小时内完全闭合。然而，在封闭条件下，体内的屏障恢复可能会延迟长达 72 小时。
Zotero 插件