Cedars Sinai Medical Center, Los Angeles, California, USA.
J Clin Microbiol. 2010 Jun;48(6):2268-70. doi: 10.1128/JCM.00166-10. Epub 2010 Mar 31.
A shortened protocol for two peptide nucleic acid fluorescence in situ hybridization (PNA FISH) assays for the detection of Gram-negative bacilli from positive blood cultures was evaluated in a multicenter trial. There was 100% concordance between the two protocols for each assay (368 of 368 and 370 of 370 results) and 99.7% (367 of 368 and 369 of 370 results) agreement with routine laboratory techniques.
一项用于检测阳性血培养物中革兰氏阴性杆菌的两种肽核酸荧光原位杂交(PNA FISH)检测法的简化方案在一项多中心试验中进行了评估。两种方案的每个检测结果均完全一致(368 个结果中的 368 个和 370 个结果中的 370 个),与常规实验室技术的一致性为 99.7%(367 个结果中的 368 个和 369 个结果中的 370 个)。