Nieto-Fernandez F, Andrieux S, Idrees S, Bagnall C, Pryor S C, Sood R
SUNY College at Old Westbury, Old Westbury, NY 11568, USA.
Invert Neurosci. 2009 Dec;9(3-4):195-200. doi: 10.1007/s10158-010-0099-5. Epub 2010 Apr 16.
Opiates modulate nociception in vertebrates. This has also been demonstrated in a number of invertebrate models. Herein, the effect of the opiate morphine and opioid neuropeptides Endomorphin 1 and 2 on the thermal avoidance (Tav) behavior of Caenorhabditis elegans is explored. Adult wild-type C. elegans N2 were collected from NGM plates using M9 buffer and exposed to morphine and endomorphine 1 and 2 in concentrations between 10(-8) and 10(-4) M (2.5 pmol/mg to 25 nmol/mg) for 30 min and tested for Tav. The opioid receptor antagonists Naloxone and CTOP were tested in combination with the drugs. Forty-seven percentage of the morphine exposed worms exhibited a class I response versus 76% of the control group (P < 0.001). Endomorphin 1 and 2 also caused a statistically significant reduction in class I responses, 36 and 39%, respectively. These effects were reversed with Naloxone and CTOP. Thermonocifensive behavior in C. elegans is modulated by opioids.
阿片类药物可调节脊椎动物的痛觉感受。这一点在许多无脊椎动物模型中也得到了证实。在此,我们探究了阿片类药物吗啡以及阿片样神经肽内吗啡肽1和内吗啡肽2对秀丽隐杆线虫热回避(Tav)行为的影响。使用M9缓冲液从NGM平板上收集成年野生型秀丽隐杆线虫N2,并将其暴露于浓度为10(-8)至10(-4) M(2.5皮摩尔/毫克至25纳摩尔/毫克)的吗啡、内吗啡肽1和内吗啡肽2中30分钟,然后测试其Tav。将阿片受体拮抗剂纳洛酮和CTOP与这些药物联合进行测试。暴露于吗啡的线虫中有47%表现出I类反应,而对照组为76%(P < 0.001)。内吗啡肽1和内吗啡肽2也分别使I类反应在统计学上显著降低,分别为36%和39%。这些作用可被纳洛酮和CTOP逆转。秀丽隐杆线虫的热防御行为受阿片类药物调节。