与小细胞肺癌相比，肺大细胞神经内分泌癌中细胞角蛋白 7、8、18、19 的高表达更为显著。

Significant high expression of cytokeratins 7, 8, 18, 19 in pulmonary large cell neuroendocrine carcinomas, compared to small cell lung carcinomas.

机构信息

Department of Molecular Diagnostics, School of Allied Health Sciences, Kitasato University, Kanagawa, Japan.

出版信息

Pathol Int. 2010 Feb;60(2):71-7. doi: 10.1111/j.1440-1827.2009.02487.x.

DOI:10.1111/j.1440-1827.2009.02487.x

PMID:20398190

Abstract

The aim of the present study was to clarify protein profiling in small cell lung carcinoma (SCLC) and pulmonary large cell neuroendocrine carcinoma (LCNEC). The proteomic approach was used, and involved cell lysate from two cell lines (N231 derived from SCLC and LCN1 derived from LCNEC), with 2-D gel electrophoresis (2-DE). In the present study, 25 protein spots with greater than twofold quantitative differences between LCN1 and N231 cells on 2-DE gels were confirmed. Within the 25 identified proteins, cytokeratins (CK) 7, 8, 18 and 19 were upregulated in LCN1 cells compared with N231 cells. The expression of CK7, 8, 18, and 19 was further studied on immunohistochemistry with 81 formalin-fixed and paraffin-embedded pulmonary carcinomas, which included 27 SCLC, 30 LCNEC, 14 adenocarcinomas, and 10 squamous cell carcinomas. Although the expression of CK7, 8, 18, and 19 was observed in all histological types, the mean immunostaining scores of CK7, 8, 18, and 19 were significantly higher in LCNEC than in SCLC (P < 0.001, P < 0.001, P < 0.01 and P < 0.001, respectively). These data suggest that the biological characteristics of LCNEC and SCLC may be different and the expression of CK may serve as differential diagnostic markers.

摘要

本研究旨在阐明小细胞肺癌（SCLC）和肺大细胞神经内分泌癌（LCNEC）的蛋白质谱。采用蛋白质组学方法，使用来自两个细胞系（源自 SCLC 的 N231 和源自 LCNEC 的 LCN1）的细胞裂解物进行 2-DE。在本研究中，通过 2-DE 凝胶证实了 25 个蛋白斑点在 LCN1 和 N231 细胞之间的定量差异大于两倍。在鉴定的 25 种蛋白质中，LCN1 细胞中细胞角蛋白（CK）7、8、18 和 19 的表达上调。用 81 例福尔马林固定和石蜡包埋的肺癌进行 CK7、8、18 和 19 的免疫组织化学进一步研究，包括 27 例 SCLC、30 例 LCNEC、14 例腺癌和 10 例鳞状细胞癌。尽管 CK7、8、18 和 19 的表达在所有组织学类型中均可见，但 CK7、8、18 和 19 的免疫染色评分在 LCNEC 中明显高于 SCLC（P<0.001、P<0.001、P<0.01 和 P<0.001）。这些数据表明 LCNEC 和 SCLC 的生物学特征可能不同，CK 的表达可能作为鉴别诊断标志物。