Analysis of redox-sensitive dynein components.

Redox-based regulation plays important roles in many cellular activities. Thioredoxins, one of the best characterized class of proteins involved in cellular redox regulation, are conserved components of eukaryotic ciliary/flagellar axonemal dyneins. Studies with Chlamydomonas showed that, under varying redox conditions, dynein-associated thioredoxins interact with different proteins through disulfide bonds and, as a consequence, flagella change their manner of beating. This chapter provides an overview of techniques for estimating and modulating the redox state of axonemal proteins, as well as for searching for redox-regulated proteins in the axoneme.