通过纳米颗粒介导的、光触发的内体破坏实现细胞不可渗透化合物的靶向细胞质递送。
Targeted cytosolic delivery of cell-impermeable compounds by nanoparticle-mediated, light-triggered endosome disruption.
机构信息
Department of Cardiology, Howard Hughes Medical Institute, Manton Center for Orphan Disease, Children's Hospital Boston, Boston, Massachusetts 02115, USA.
出版信息
Nano Lett. 2010 Jun 9;10(6):2211-9. doi: 10.1021/nl101157z.
Abstract
Nanoparticle (NP)-mediated drug delivery typically relies on cargo release to occur passively or in response to environmental stimuli. Here we present a delivery method based on light-activated disruption of intracellular vesicles after internalization of biofunctionalized mesoporous silica nanoparticles loaded with cargo. This method combines the power of targeted delivery with the spatiotemporal control of light activation. As an example, we delivered a cell-impermeable fluorescent compound exclusively to the cytosol of multidrug resistant cancer cells in a mixed population.
摘要
纳米颗粒（NP）介导的药物输送通常依赖于货物的被动释放或对环境刺激的响应。在这里，我们提出了一种基于生物功能化介孔二氧化硅纳米颗粒负载货物内化后用光激活破坏细胞内囊泡的递药方法。该方法将靶向递药的威力与光激活的时空控制相结合。例如，我们将一种细胞不可渗透的荧光化合物递送到混合群体中的多药耐药癌细胞的细胞质中。
相似文献
1
Targeted cytosolic delivery of cell-impermeable compounds by nanoparticle-mediated, light-triggered endosome disruption.
Nano Lett. 2010 Jun 9;10(6):2211-9. doi: 10.1021/nl101157z.
2
Cytosolic delivery of LDL nanoparticle cargo using photochemical internalization.
Photochem Photobiol Sci. 2011 May;10(5):810-6. doi: 10.1039/c0pp00350f. Epub 2011 Feb 23.
3
New Mechanism for Release of Endosomal Contents: Osmotic Lysis via Nigericin-Mediated K/H Exchange.
Bioconjug Chem. 2018 Apr 18;29(4):1047-1059. doi: 10.1021/acs.bioconjchem.7b00714. Epub 2018 Mar 2.
4
TAT and HA2 facilitate cellular uptake of gold nanoparticles but do not lead to cytosolic localisation.
PLoS One. 2015 Apr 2;10(4):e0121683. doi: 10.1371/journal.pone.0121683. eCollection 2015.
5
Optical and electron microscopy study of laser-based intracellular molecule delivery using peptide-conjugated photodispersible gold nanoparticle agglomerates.
J Nanobiotechnology. 2016 Jan 8;14:2. doi: 10.1186/s12951-015-0155-8.
6
Endosome-disruptive peptides for improving cytosolic delivery of bioactive macromolecules.
Biopolymers. 2010;94(6):763-70. doi: 10.1002/bip.21487.
7
A quantitative study of the intracellular fate of pH-responsive doxorubicin-polypeptide nanoparticles.
J Control Release. 2017 Aug 28;260:100-110. doi: 10.1016/j.jconrel.2017.05.032. Epub 2017 May 30.
8
Exploiting lipid raft transport with membrane targeted nanoparticles: a strategy for cytosolic drug delivery.
Biomaterials. 2008 Aug;29(23):3367-75. doi: 10.1016/j.biomaterials.2008.04.030. Epub 2008 May 16.
9
Photochemical internalisation: a novel drug delivery system.
Tumour Biol. 2002 Mar-Apr;23(2):103-12. doi: 10.1159/000059713.
10
Porous Silicon Nanoneedles Modulate Endocytosis to Deliver Biological Payloads.
Adv Mater. 2019 Mar;31(12):e1806788. doi: 10.1002/adma.201806788. Epub 2019 Jan 24.
引用本文的文献
1
A Comprehensive Review: Mesoporous Silica Nanoparticles Greatly Improve Pharmacological Effectiveness of Phytoconstituent in Plant Extracts.
Pharmaceuticals (Basel). 2024 Dec 13;17(12):1684. doi: 10.3390/ph17121684.
2
Targeting the Inside of Cells with Biologicals: Chemicals as a Delivery Strategy.
BioDrugs. 2021 Nov;35(6):643-671. doi: 10.1007/s40259-021-00500-y. Epub 2021 Oct 27.
3
Advances in Functionalized Photosensitive Polymeric Nanocarriers.
Polymers (Basel). 2021 Jul 27;13(15):2464. doi: 10.3390/polym13152464.
4
An Update on Mesoporous Silica Nanoparticle Applications in Nanomedicine.
Pharmaceutics. 2021 Jul 12;13(7):1067. doi: 10.3390/pharmaceutics13071067.
5
Engineered Polymeric Materials for Biological Applications: Overcoming Challenges of the Bio-Nano Interface.
Polymers (Basel). 2019 Sep 2;11(9):1441. doi: 10.3390/polym11091441.
6
Antibody-Drug Conjugate that Exhibits Synergistic Cytotoxicity with an Endosome-Disruptive Peptide.
ACS Omega. 2019 Jul 31;4(7):12955-12968. doi: 10.1021/acsomega.9b01585.
7
Mesoporous Silica Nanoparticles: A Comprehensive Review on Synthesis and Recent Advances.
Pharmaceutics. 2018 Aug 6;10(3):118. doi: 10.3390/pharmaceutics10030118.
8
Protein delivery into cells using inorganic nanoparticle-protein supramolecular assemblies.
Chem Soc Rev. 2018 May 21;47(10):3421-3432. doi: 10.1039/c8cs00008e.
9
Polysaccharide-based nanoparticles for co-loading mitoxantrone and verapamil to overcome multidrug resistance in breast tumor.
Int J Nanomedicine. 2017 Oct 10;12:7337-7350. doi: 10.2147/IJN.S145620. eCollection 2017.
10
A DNA dual lock-and-key strategy for cell-subtype-specific siRNA delivery.
Nat Commun. 2016 Nov 24;7:13580. doi: 10.1038/ncomms13580.
本文引用的文献
1
Enzyme responsive nanocontainers with cyclodextrin gatekeepers and synergistic effects in release of guests.
J Am Chem Soc. 2009 Nov 25;131(46):16614-5. doi: 10.1021/ja9061085.
2
Targeted intracellular delivery of hydrophobic agents using mesoporous hybrid silica nanoparticles as carrier systems.
Nano Lett. 2009 Sep;9(9):3308-11. doi: 10.1021/nl901589y.
3
Photochemical internalization (PCI) in cancer therapy: from bench towards bedside medicine.
J Photochem Photobiol B. 2009 Aug 3;96(2):83-92. doi: 10.1016/j.jphotobiol.2009.04.012. Epub 2009 May 8.
4
Structure of P-glycoprotein reveals a molecular basis for poly-specific drug binding.
Science. 2009 Mar 27;323(5922):1718-22. doi: 10.1126/science.1168750.
5
Light-sensitive intelligent drug delivery systems.
Photochem Photobiol. 2009 Jul-Aug;85(4):848-60. doi: 10.1111/j.1751-1097.2008.00530.x. Epub 2009 Feb 13.
6
Molecular basis of multidrug transport by ABC transporters.
Biochim Biophys Acta. 2009 May;1794(5):725-37. doi: 10.1016/j.bbapap.2008.12.004. Epub 2008 Dec 24.
7
A general route to diverse mesoporous metal oxide submicrospheres with highly crystalline frameworks.
Angew Chem Int Ed Engl. 2008;47(45):8682-6. doi: 10.1002/anie.200802487.
8
Nanoparticle therapeutics: an emerging treatment modality for cancer.
Nat Rev Drug Discov. 2008 Sep;7(9):771-82. doi: 10.1038/nrd2614.
9
Seven challenges for nanomedicine.
Nat Nanotechnol. 2008 May;3(5):242-4. doi: 10.1038/nnano.2008.114.
10
Nanoparticle-mediated cellular response is size-dependent.
Nat Nanotechnol. 2008 Mar;3(3):145-50. doi: 10.1038/nnano.2008.30. Epub 2008 Mar 2.
Zotero 插件