Equipe Neurosciences Pharmacologie et Environnement, and Centre National d'Études et de Recherches sur le Sahara (CNERS), Université Cadi Ayyad, Faculté des Sciences Semlalia, Marrakech, Morocco.
Acta Histochem. 2011 May;113(3):369-74. doi: 10.1016/j.acthis.2010.01.011. Epub 2010 May 5.
Proopiomelanocortin (POMC) is a 36kDa glycoprotein implicated in homeostatic balance. We used in situ hybridization histochemistry coupled with quantitative autoradiography to determine the anatomical distribution of POMC mRNA-expressing neurons in the arcuate nucleus (AN) and to examine the effects of prolonged dehydration on POMC gene expression in a semi-desert rodent, Meriones shawi (Shaw's Jird). In the hypothalamus of control animals, POMC mRNA-expressing neurons were exclusively localized in the AN and they showed a differential distribution and density along its rostro-caudal subdivisions. In dehydrated animals, water deprivation caused a decrease in POMC mRNA labeling in the AN. These results suggest that dehydration stress can induce negative regulation of POMC gene expression in this species. A comparative study of weight variation between control and dehydrated animals showed a weight loss followed by stabilization of weight during prolonged dehydration.
促黑皮质素原（POMC）是一种 36kDa 的糖蛋白，与体内平衡有关。我们采用原位杂交组织化学结合定量放射自显影技术，确定了 POMC mRNA 表达神经元在弓状核（AN）中的解剖分布，并研究了长期脱水对沙氏仓鼠（Shaw's Jird）POMC 基因表达的影响。在对照动物的下丘脑，POMC mRNA 表达神经元仅定位于 AN，并且它们在其前后轴部分化中表现出不同的分布和密度。在脱水动物中，水剥夺导致 AN 中 POMC mRNA 标记减少。这些结果表明，脱水应激可以诱导该物种 POMC 基因表达的负调控。对对照和脱水动物体重变化的比较研究表明，在长时间脱水过程中，体重先下降后稳定。
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