Laboratory of Biochemistry, UR03/ ES-08 Human Nutrition and Metabolic Disorders, Faculty of Medicine, Monastir 5019, Tunisia.
J Hazard Mater. 2010 Aug 15;180(1-3):225-33. doi: 10.1016/j.jhazmat.2010.04.018. Epub 2010 Apr 14.
"Désormone Lourd" is a 2,4-Dichlorophenoxyacetic based herbicide that includes 600 g/L 2,4-D. In this study we analyzed the toxic effects of 2,4-D on rat liver. Animals were daily treated with 15, 75 and 150 mg/kg, via oral gavage during 4 weeks. Hepatotoxicity was monitored by quantitative analysis of the serum enzymes markers of hepatotoxicity. Oxidative stress markers, catalase and glutathione reductase (CAT and GR), were analyzed in liver. We also investigated liver tissues histopathologically. Our results revealed that, when rats of 2,4-D treated groups were compared with the control group, the body weight decreased and the liver weight increased significantly at the end of the 4th week. The microscopic evaluation showed that 2,4-D induced hepatic cord disruption, focal necrosis, vessel dilation and pycnotic nucleus. Histological effects were found in all treated groups and their severity was dose dependent. Through sub-acute treatment, starting from the low to the high doses of 2,4-D, it was observed that there were effects on the activity of the serum enzyme markers, on TSP, Alb and the glycemia levels. We also observed a significant reduction in the hepatic antioxidant enzyme activities. To conclude, we can suggest that 2,4-D induces hepatoxicity and cellular alterations in rat.
“Désormone Lourd”是一种基于 2,4-二氯苯氧乙酸的除草剂，包含 600 g/L 的 2,4-D。在本研究中，我们分析了 2,4-D 对大鼠肝脏的毒性作用。动物通过口服灌胃，每天接受 15、75 和 150 mg/kg 的剂量，连续 4 周。通过定量分析血清肝毒性标志物来监测肝毒性。在肝脏中分析氧化应激标志物，包括过氧化氢酶（CAT）和谷胱甘肽还原酶（GR）。我们还对肝组织进行了组织病理学检查。结果显示，与对照组相比，2,4-D 处理组的大鼠在第 4 周结束时体重下降，肝脏重量显著增加。显微镜评估显示，2,4-D 诱导肝索中断、局灶性坏死、血管扩张和核固缩。所有处理组均出现组织学效应，其严重程度与剂量有关。通过亚急性处理，从低剂量到高剂量的 2,4-D，观察到血清酶标志物、TSP、Alb 和血糖水平的活性发生变化。还观察到肝抗氧化酶活性显著降低。综上所述，我们可以认为 2,4-D 会引起大鼠肝毒性和细胞改变。
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