Department of Laboratory Medicine, Molecular Diagnosis Centre, National University Hospital, Singapore.
Ann Acad Med Singap. 2010 Apr;39(4):291-4.
Since the emergence of the pandemic influenza A/H1N1/2009 virus in April 2009, diagnostic testing in many countries has revealed the rapid displacement and then replacement of circulating seasonal influenza viruses by this novel virus.
In-house seasonal and pandemic influenza-specific polymerase chain reaction assays were introduced and/or developed at the Molecular Diagnosis Centre (MDC) at the National University Hospital (NUH), Singapore. These assays have been used to test all samples received from in-patients, out-patients, staff and visitors for suspected pandemic influenza A/H1N1/2009 infection.
Prior to the arrival of the pandemic A/H1N1/2009 virus in Singapore at the end of May 2009, seasonal influenza A/H3N2 predominated in this population, with very little seasonal influenza A/H1N1 and B viruses detected. Within about 1 month of its arrival in Singapore (mainly during June to July 2009), this pandemic virus rapidly displaced seasonal influenza A/H3N2 to become the predominant strain in the Singaporean population served by MDC/NUH.
Realtime molecular techniques have allowed the prompt detection of different influenza subtypes during this current pandemic, which has revealed the displacement/replacement of previously circulating seasonal subtypes with A/H1N1/2009. Although some of this may be explained by immunological cross-reactivity between influenza subtypes, more studies are required.
自 2009 年 4 月大流行性甲型 H1N1/2009 流感病毒出现以来，许多国家的诊断检测表明，这种新型病毒迅速取代了循环季节性流感病毒。
在新加坡国立大学医院（NUH）分子诊断中心（MDC）引入和/或开发了季节性和大流行性流感特异性聚合酶链反应检测。这些检测已用于测试所有来自住院患者、门诊患者、工作人员和访客的疑似大流行性甲型 H1N1/2009 感染的样本。
在 2009 年 5 月底大流行性 A/H1N1/2009 病毒抵达新加坡之前，该人群中季节性甲型 H3N2 占主导地位，很少检测到季节性甲型 H1N1 和 B 病毒。在它抵达新加坡后大约 1 个月内（主要在 2009 年 6 月至 7 月期间），这种大流行性病毒迅速取代了季节性甲型 H3N2，成为 MDC/NUH 服务的新加坡人群中的主要菌株。
实时分子技术允许在当前大流行期间快速检测到不同的流感亚型，这揭示了以前循环的季节性亚型与 A/H1N1/2009 的替代/替代。虽然这可能部分归因于流感亚型之间的免疫交叉反应，但需要进行更多的研究。
