可溶性小鼠白细胞介素-6受体及抗小鼠白细胞介素-6受体抗体的制备。
Preparation of soluble murine IL-6 receptor and anti-murine IL-6 receptor antibodies.
作者信息
Saito T, Yasukawa K, Suzuki H, Futatsugi K, Fukunaga T, Yokomizo C, Koishihara Y, Fukui H, Ohsugi Y, Yawata H
机构信息
Biotechnology Research Laboratory, Tosoh Corporation, Kanagawa, Japan.
出版信息
J Immunol. 1991 Jul 1;147(1):168-73.
Starting with a previously isolated cDNA clone encoding murine IL-6R, a stable transformed Chinese hamster ovary cell line constitutively expressing soluble murine IL-6R (smIL-6R) has been established. The smIL-6R was purified to homogeneity by sequential filtration and chromatography of culture medium. The smIL-6R augmented the sensitivity of M1 cells to IL-6 in their growth inhibition in a dose-response manner. Rat hybridomas producing mAb specific to murine IL-6R were also established. One of the clones, RS13, produced IgG2a isotype that was capable of inhibiting IL-6 activity. ELISA for the quantitation of smIL-6R was established, which could detect smIL-6R in a quantity as low as 1 ng/ml.
从先前分离的编码小鼠白细胞介素-6受体（IL-6R）的cDNA克隆开始，已建立了一个稳定转化的组成型表达可溶性小鼠IL-6R（smIL-6R）的中国仓鼠卵巢细胞系。通过对培养基进行连续过滤和层析，将smIL-6R纯化至同质。smIL-6R以剂量反应方式增强了M1细胞在生长抑制中对IL-6的敏感性。还建立了产生针对小鼠IL-6R的单克隆抗体（mAb）的大鼠杂交瘤。其中一个克隆RS13产生能够抑制IL-6活性的IgG2a同种型。建立了用于定量smIL-6R的酶联免疫吸附测定（ELISA），其能够检测低至1 ng/ml量的smIL-6R。
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