对来自市政、医院和二级处理设施的污水中的抗微生物药物耐药性大肠杆菌的计数和特性分析。
Enumeration and characterization of antimicrobial-resistant Escherichia coli bacteria in effluent from municipal, hospital, and secondary treatment facility sources.
机构信息
Department of Bacteriology, Clinical Science Institute, National University of Ireland, Galway, Ireland.
出版信息
Appl Environ Microbiol. 2010 Jul;76(14):4772-9. doi: 10.1128/AEM.02898-09. Epub 2010 Jun 4.
Abstract
We describe a modification of the most probable number (MPN) method for rapid enumeration of antimicrobial-resistant Escherichia coli bacteria in aqueous environmental samples. E. coli (total and antimicrobial-resistant) bacteria were enumerated in effluent samples from a hospital (n = 17) and municipal sewers upstream (n = 5) and downstream (n = 5) from the hospital, effluent samples from throughout the treatment process (n = 4), and treated effluent samples (n = 13). Effluent downstream from the hospital contained a higher proportion of antimicrobial-resistant E. coli than that upstream from the hospital. Wastewater treatment reduced the numbers of E. coli bacteria (total and antimicrobial resistant); however, antimicrobial-resistant E. coli was not eliminated, and E. coli resistant to cefotaxime (including extended-spectrum beta-lactamase [ESBL] producers), ciprofloxacin, and cefoxitin was present in treated effluent samples.
摘要
我们描述了一种改良的最可能数（MPN）方法，用于快速检测水环境样品中的抗菌耐药大肠杆菌。在医院废水（n = 17）和城市下水道上游（n = 5）和下游（n = 5）、整个处理过程的废水样品（n = 4）和处理后的废水样品（n = 13）中对大肠杆菌（总大肠杆菌和抗菌耐药大肠杆菌）进行了计数。医院下游的废水中含有更高比例的抗菌耐药大肠杆菌。废水处理减少了大肠杆菌的数量（总大肠杆菌和抗菌耐药大肠杆菌）；然而，抗菌耐药大肠杆菌并未被消除，并且在处理后的废水样品中存在对头孢噻肟（包括超广谱β-内酰胺酶 [ESBL]产生菌）、环丙沙星和头孢西丁耐药的大肠杆菌。
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