[使用不同染色技术对人芽囊原虫诊断进行标准化]
[Standardization of Blastocystis hominis diagnosis using different staining techniques].
作者信息
Eymael Dayane, Schuh Graziela Maria, Tavares Rejane Giacomelli
机构信息
Laboratório de Biomedicina, Setor de Parasitologia, Universidade Feevale, Novo Hamburgo, RS.
出版信息
Rev Soc Bras Med Trop. 2010 May-Jun;43(3):309-12. doi: 10.1590/s0037-86822010000300019.
INTRODUCTION
The present study was carried out from March to May 2008, with the aim of evaluating the effectiveness of different techniques for diagnosing Blastocystis hominis in a sample of the population attended at the Biomedicine Laboratory of Feevale University, Novo Hamburgo, Rio Grande do Sul.
METHODS
On hundred feces samples from children and adults were evaluated. After collection, the samples were subjected to the techniques of spontaneous sedimentation (HPJ), sedimentation in formalin-ether (Ritchie) and staining by means of Gram and May-Grünwald-Giemsa (MGG).
RESULTS
The presence of Blastocystis hominis was observed in 40 samples, when staining techniques were used (MGG and Gram), while sedimentation techniques were less efficient (32 positive samples using the Ritchie technique and 20 positive samples using the HPJ technique).
CONCLUSIONS
Our results demonstrate that HPJ was less efficient than the other methods, thus indicating the need to include laboratory techniques that enable parasite identification on a routine basis.
引言
本研究于2008年3月至5月开展，旨在评估不同技术在诊断南里奥格兰德州新汉堡费瓦莱大学生物医学实验室就诊人群样本中人体芽囊原虫的有效性。
方法
对100份儿童和成人粪便样本进行评估。样本采集后，采用自然沉淀法（HPJ）、福尔马林-乙醚沉淀法（Ritchie）以及革兰氏染色和迈-格-姬姆萨染色法（MGG）进行检测。
结果
采用染色技术（MGG和革兰氏染色）时，在40份样本中检测到人体芽囊原虫，而沉淀技术的效率较低（Ritchie技术检测出32份阳性样本，HPJ技术检测出20份阳性样本）。
结论
我们的结果表明，HPJ技术比其他方法效率更低，因此表明需要纳入能够在常规基础上进行寄生虫鉴定的实验室技术。
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