用于DNA核磁共振研究的立体选择性核苷氘代

Stereoselective nucleoside deuteration for NMR studies of DNA.

作者信息

Lukin Mark, de los Santos Carlos

机构信息

Department of Pharmacological Sciences, Stony Brook University, Stony Brook, New York, USA.

出版信息

Nucleosides Nucleotides Nucleic Acids. 2010 Jul;29(7):562-73. doi: 10.1080/15257770.2010.494650.

DOI:10.1080/15257770.2010.494650

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3836267/

Abstract

A procedure has been elaborated for stereoselective deuterium substitution of one of the diastereotopic 5'-protons in 2'-deoxynucleotides. The synthetic scheme uses the reduction of the 5-oxosugar derivative with deuterated Alpine-Borane. The resulting deuterosugar is converted into pyrimidine nucleosides and incorporated into DNA using standard protocols. Comparison of two-dimensional NMR spectra of the fully protonated and partially deuterated duplexes allowed us to assign diastereotopic 5' protons, increasing the number of experimental restraints used for structure determination.

摘要

已经精心设计了一种用于对2'-脱氧核苷酸中两个非对映异位5'-质子之一进行立体选择性氘代的方法。该合成方案利用氘代高山硼烷还原5-氧代糖衍生物。所得的氘代糖被转化为嘧啶核苷，并使用标准方案掺入DNA中。通过比较完全质子化和部分氘代双链体的二维核磁共振谱，我们能够确定非对映异位的5'质子，从而增加了用于结构测定的实验约束数量。

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