从有限数量的造血祖细胞中得出的全基因组染色质图谱。
Genome-wide chromatin maps derived from limited numbers of hematopoietic progenitors.
机构信息
Howard Hughes Medical Institute, Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
出版信息
Nat Methods. 2010 Aug;7(8):615-8. doi: 10.1038/nmeth.1478. Epub 2010 Jul 11.
Abstract
Current methods for whole-genome mapping of protein-DNA interactions, performed by coupling chromatin immunoprecipitation with high-throughput sequencing (ChIP-seq), require large amounts of starting materials, which precludes their application to rare cell types. Here we combine a high-sensitivity ChIP assay with a new library preparation procedure to map histone modifications in as few as 10,000 cells. We used the technique to characterize mouse hematopoietic progenitors and thereby gain insight into their developmental program.
摘要
目前，通过将染色质免疫沉淀与高通量测序（ChIP-seq）相结合来进行蛋白质-DNA 相互作用的全基因组作图的方法需要大量的起始材料，这使得它们无法应用于稀有细胞类型。在这里，我们将高灵敏度的 ChIP 测定法与一种新的文库制备程序相结合，以便在仅 10000 个细胞中绘制组蛋白修饰图谱。我们使用该技术对小鼠造血祖细胞进行了特征描述，从而深入了解了它们的发育程序。
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