利用粪便标本进行环介导等温扩增法检测人体中带绦虫种属的差异。
Evaluation of a loop-mediated isothermal amplification method using fecal specimens for differential detection of Taenia species from humans.
机构信息
Department of Parasitology, Asahikawa Medical College, Midorigaoka Higashi 2-1-1-1, Asahikawa 078-8510, Hokkaido, Japan.
出版信息
J Clin Microbiol. 2010 Sep;48(9):3350-2. doi: 10.1128/JCM.00697-10. Epub 2010 Jul 14.
Abstract
We compared the performance of loop-mediated isothermal amplification (LAMP) with that of a multiplex PCR method for differential detection of human Taenia parasites in fecal specimens from taeniasis patients. The LAMP method, with no false positives, showed a higher sensitivity (88.4%) than the multiplex PCR (37.2%). Thus, it is expected that the LAMP method has a high value for molecular diagnosis of taeniasis.
摘要
我们比较了环介导等温扩增（LAMP）与多重 PCR 方法在检测粪便样本中人类带绦虫寄生虫的差异方面的性能。LAMP 方法没有假阳性，显示出比多重 PCR 更高的敏感性（88.4%）。因此，预计 LAMP 方法在带绦虫病的分子诊断方面具有很高的价值。
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Infect Genet Evol. 2010 May;10(4):444-52. doi: 10.1016/j.meegid.2010.01.011. Epub 2010 Feb 2.
2
Development of a species-specific coproantigen ELISA for human Taenia solium taeniasis.
Am J Trop Med Hyg. 2009 Sep;81(3):433-7.
3
Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases.
J Infect Chemother. 2009 Apr;15(2):62-9. doi: 10.1007/s10156-009-0669-9. Epub 2009 Apr 25.
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J Clin Microbiol. 2009 Jun;47(6):1892-5. doi: 10.1128/JCM.00105-09. Epub 2009 Mar 25.
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6
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J Clin Microbiol. 2008 Jan;46(1):286-9. doi: 10.1128/JCM.01172-07. Epub 2007 Nov 7.
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