[四种常见牙周病原体体外侵袭血管内皮细胞的研究]

[Invasion of four common periodontal pathogens into vascular endothelial cells in vitro].

作者信息

Deng Hui, Wu Ya-fei, Ding Yi, Miao Di, Gao Li, Guo Shu-juan

机构信息

Department of Periodontology, West China School of Stomatology, Sichuan University, Chengdu 610041, China.

出版信息

Zhonghua Kou Qiang Yi Xue Za Zhi. 2010 Apr;45(4):203-6.

PMID:20654193

Abstract

OBJECTIVE

To investigate the adhesive and invasive ability of four common periodontal pathogens, Pg33277, Pi25611, Aa29522 and Fn10953 in human umbilical vein endothelial cells (HUVEC).

METHODS

The model of infection of HUVEC by periodontal pathogens was established in vitro. The invasive ability of four periodontal pathogens in HUVEC was tested by scanning electron microscope (SEM) and antibiotic protection assays-colony-forming units (CFU).

RESULTS

All of the four periodontal pathogens were found to adhere to HUVEC by SEM and invaded HUVEC at invasion numbers of (0.8 +/- 0.1) x 10(8), (4.1 +/- 0.5) x 10(6), (1.6 +/- 0.3) x 10(6) and (5.0 +/- 0.4) x 10(6) CFU/L respectively by antibiotic protection assays-CFU. The invasion efficiencies were (0.400 +/- 0.050)%, (0.021 +/- 0.003)%, (0.008 +/- 0.002)% and (0.025 +/- 0.002)%, respectively. The invasive ability of Pg33277 was significantly greater than those of the other three periodontal pathogens (P < 0.001). There was no difference in invasive abilities among Pi25611, Aa29522 and Fn10953 (P > 0.05).

CONCLUSIONS

All of the four common periodontal pathogens, Pg33277, Pi25611, Aa29522 and Fn10953 could adhere to and invaded HUVEC, with Pg33277 being the strongest.

摘要

目的

研究四种常见牙周病原体Pg33277、Pi25611、Aa29522和Fn10953对人脐静脉内皮细胞（HUVEC）的黏附及侵袭能力。

方法

体外建立牙周病原体感染HUVEC的模型。采用扫描电子显微镜（SEM）及抗生素保护试验-菌落形成单位（CFU）检测四种牙周病原体对HUVEC的侵袭能力。

结果

SEM观察发现四种牙周病原体均可黏附于HUVEC，抗生素保护试验-CFU检测其侵袭HUVEC的数量分别为(0.8±0.1)×10⁸、(4.1±0.5)×10⁶、(1.6±0.3)×10⁶和(5.0±0.4)×10⁶CFU/L。侵袭效率分别为(0.400±0.050)%、(0.021±0.003)%、(0.008±0.002)%和(0.025±0.002)%。Pg33277的侵袭能力显著强于其他三种牙周病原体（P<0.001）。Pi25611、Aa29522和Fn10953之间的侵袭能力无差异（P>0.05）。