果蝇中肠干细胞和成肠细胞特异性Gal4系的表征
Characterization of midgut stem cell- and enteroblast-specific Gal4 lines in drosophila.
作者信息
Zeng Xiankun, Chauhan Chhavi, Hou Steven X
机构信息
Mouse Cancer Genetics Program, National Cancer Institute at Frederick, National Institutes of Health, Frederick, Maryland, USA.
出版信息
Genesis. 2010 Oct 1;48(10):607-11. doi: 10.1002/dvg.20661.
Abstract
The homeostasis of Drosophila midgut is maintained by multipotent intestinal stem cells (ISCs), each of which gives rise to a new ISC and an immature daughter cell, enteroblast (EB), after one asymmetric cell division. In Drosophila, the Gal4-UAS system is widely used to manipulate gene expression in a tissue- or cell-specific manner, but in Drosophila midgut, there are no ISC- or EB-specific Gal4 lines available. Here we report the generation and characterization of Dl-Gal4 and Su(H)GBE-Gal4 lines, which are expressed specifically in the ISCs and EBs separately. Additionally, we demonstrate that Dl-Gal4 and Su(H)GBE-Gal4 are expressed in adult midgut progenitors (AMPs) and niche peripheral cells (PCs) separately in larval midgut. These two Gal4 lines will serve as invaluable tools for navigating ISC behaviors.
摘要
果蝇中肠的稳态由多能肠干细胞（ISC）维持，每个ISC在一次不对称细胞分裂后会产生一个新的ISC和一个未成熟的子细胞，即成肠细胞（EB）。在果蝇中，Gal4-UAS系统被广泛用于以组织或细胞特异性方式操纵基因表达，但在果蝇中肠中，没有可用的ISC或EB特异性Gal4品系。在这里，我们报告了Dl-Gal4和Su(H)GBE-Gal4品系的产生和特性，它们分别在ISC和EB中特异性表达。此外，我们证明Dl-Gal4和Su(H)GBE-Gal4在幼虫中肠中分别在成年中肠祖细胞（AMP）和微环境外周细胞（PC）中表达。这两个Gal4品系将成为研究ISC行为的宝贵工具。
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本文引用的文献
1
Transcriptional control of stem cell maintenance in the Drosophila intestine.
Development. 2010 Mar;137(5):705-14. doi: 10.1242/dev.039404.
2
JAK-STAT is restrained by Notch to control cell proliferation of the Drosophila intestinal stem cells.
J Cell Biochem. 2010 Apr 1;109(5):992-9. doi: 10.1002/jcb.22482.
3
A transient niche regulates the specification of Drosophila intestinal stem cells.
Science. 2010 Jan 8;327(5962):210-3. doi: 10.1126/science.1181958.
4
JAK/STAT signaling coordinates stem cell proliferation and multilineage differentiation in the Drosophila intestinal stem cell lineage.
Dev Biol. 2010 Feb 1;338(1):28-37. doi: 10.1016/j.ydbio.2009.10.045. Epub 2009 Nov 6.
5
Paracrine unpaired signaling through the JAK/STAT pathway controls self-renewal and lineage differentiation of drosophila intestinal stem cells.
J Mol Cell Biol. 2010 Feb;2(1):37-49. doi: 10.1093/jmcb/mjp028. Epub 2009 Sep 30.
6
Regulation of intestinal stem cells in mammals and Drosophila.
J Cell Physiol. 2010 Jan;222(1):33-7. doi: 10.1002/jcp.21928.
7
Cytokine/Jak/Stat signaling mediates regeneration and homeostasis in the Drosophila midgut.
Cell. 2009 Jun 26;137(7):1343-55. doi: 10.1016/j.cell.2009.05.014.
8
Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.
Science. 2009 Jul 17;325(5938):340-3. doi: 10.1126/science.1173164. Epub 2009 Jun 11.
9
Pathogenic stimulation of intestinal stem cell response in Drosophila.
J Cell Physiol. 2009 Sep;220(3):664-71. doi: 10.1002/jcp.21808.
10
Drosophila intestinal response to bacterial infection: activation of host defense and stem cell proliferation.
Cell Host Microbe. 2009 Feb 19;5(2):200-11. doi: 10.1016/j.chom.2009.01.003.
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