Department of Biology, Center for Integrated Protein Science Munich, Ludwig Maximilians University Munich, 82152 Planegg-Martinsried, Germany.
Nucleic Acids Res. 2010 Oct;38(19):e181. doi: 10.1093/nar/gkq684. Epub 2010 Aug 4.
The recent discovery of genomic 5-hydroxymethylcytosine (hmC) and mutations affecting the respective Tet hydroxylases in leukemia raises fundamental questions about this epigenetic modification. We present a sensitive method for fast quantification of genomic hmC based on specific transfer of radiolabeled glucose to hmC by a purified glucosyltransferase. We determined hmC levels in various adult tissues and differentiating embryonic stem cells and show a correlation with differential expression of tet genes.
最近在白血病中发现了基因组 5-羟甲基胞嘧啶（hmC）和影响相应 Tet 羟化酶的突变，这引发了关于这种表观遗传修饰的基本问题。我们提出了一种基于纯化的葡萄糖基转移酶将放射性标记的葡萄糖特异性转移到 hmC 上来快速定量基因组 hmC 的灵敏方法。我们测定了各种成人组织和分化的胚胎干细胞中的 hmC 水平，并显示与 tet 基因的差异表达相关。
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