将半乳糖配体固定在温敏培养表面及其对细胞黏附/脱落的影响。

Immobilization of galactose ligands on thermoresponsive culture surface and its influence on cell adhesion/detachment.

机构信息

Tianjin Key Laboratory of Fiber Modification and Functional Fiber, School of Materials Science and Engineering, Tianjin Polytechnic University, Tianjin 300160, China.

出版信息

J Colloid Interface Sci. 2010 Oct 15;350(2):471-9. doi: 10.1016/j.jcis.2010.07.013. Epub 2010 Jul 13.

DOI:10.1016/j.jcis.2010.07.013

PMID:20691452

Abstract

A novel bio-functionalized thermoresponsive surface was prepared by UV-induced copolymerization of N-isopropylacrylamide (NIPAAm) and acrylic acid (AAc) and grafting onto tissue culture polystyrene (TCPS) dishes, followed by immobilization of galactose ligands. The results indicated that the roughness of surfaces was increased after copolymerization with NIPAAm and immobilization of galactose ligands. The amount of monomers grafted on TCPS was increased with increasing photografting time. The temperature sensitivity of surface was improved with increasing amount of NIPAAm grafted on TCPS, and surface hydrophilicity was enhanced by the introduction of carboxyl groups and galactose ligands, which accelerated cell detachment. Adhesion, proliferation, detachment and transshipment of human hepatoma cell line (HepG2) on the modified surfaces were examined. The immobilization of galactose ligands facilitated the cell adhesion and proliferation. HepG2 cells growing on the modified surfaces could be recovered spontaneously by only reducing culture temperature. The activity of cells obtained by temperature induction was higher than that obtained by enzymatic digestion.

摘要

一种新型的生物功能化温敏表面通过 N-异丙基丙烯酰胺（NIPAAm）和丙烯酸（AAc）的紫外光共聚以及接枝到组织培养聚苯乙烯（TCPS）盘上，并随后固定半乳糖配体而制备。结果表明，与 NIPAAm 共聚以及固定半乳糖配体后，表面的粗糙度增加。在 TCPS 上接枝的单体数量随光接枝时间的增加而增加。在 TCPS 上接枝的 NIPAAm 数量增加，表面的温度敏感性得到改善，并且通过引入羧基和半乳糖配体提高了表面的亲水性，从而加速了细胞脱落。在修饰的表面上，人肝癌细胞系（HepG2）的粘附、增殖、脱落和转运被检测到。半乳糖配体的固定促进了细胞的粘附和增殖。在仅降低培养温度的情况下，生长在修饰表面上的 HepG2 细胞可以自发恢复。通过温度诱导获得的细胞的活性高于通过酶消化获得的细胞的活性。