利用二氢叶酸还原酶-胸苷酸合成酶连接区准确、敏感地检测人类中的疟原虫种。
Accurate and sensitive detection of Plasmodium species in humans by use of the dihydrofolate reductase-thymidylate synthase linker region.
机构信息
Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
出版信息
J Clin Microbiol. 2010 Oct;48(10):3735-7. doi: 10.1128/JCM.00898-10. Epub 2010 Aug 11.
Abstract
A nested-PCR protocol based on the linker region of the Plasmodium dihydrofolate reductase-thymidylate synthase gene (dhfr-ts) was developed. This provides highly sensitive specific detection and identification of the five parasite species that infect humans.
摘要
建立了一种基于疟原虫二氢叶酸还原酶-胸苷酸合成酶基因（dhfr-ts）连接区的巢式 PCR 方法。该方法可高度敏感和特异地检测和鉴定感染人类的 5 种疟原虫。
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