通过ISSR-PCR分析评估所选苹果（苹果属杂交种Borkh.）品种的遗传变异性。

Evaluation of genetic variability in choosen apple (Malus x domestica Borkh.) cultivars by ISSR-PCR analysis.

作者信息

Smolik M, Krzysztoszek O

机构信息

Department of Horticultural Plant Breeding, West Pomeranian University of Technology, Szczecin 71-424, Poland.

出版信息

Genetika. 2010 Jul;46(7):923-31.

PMID:20795496

Abstract

The aim of the study was to determine the genetic variability in eight apple cultivars: Delikates, Cortland, James Grieve, Lired, Jonathan, Golden Delicious, Jonagold and Idared from the collection of Fruit Growing Research Station in Rajkowo of the West Pomeranian University of Technology, Szczecin. The cultivar Delikates was obtained from the crossing of two cultivars: Cortland and James Grieve, whereas cultivar Lired is a James Grieve's sport. The second one cultivar--Jonagold was obtained from the crossing of Jonathan and Golden Delicious. The cultivar Idared is a hybrid obtained from the crossing of Jonathan and Wagener. Out of 40 primers, 17 were chosen for the final study. Those amplified a total of 183 loci (872 amplicons) out of which 34 (18.5%) were monomorphic, 128 (69.5%) were polymorphic and 22 (12%) cultivar-specific. Specific ISSR products were detected for each apple cultivar. A dendrogram was constructed using the UPGMA method which revealed two distinct clusters: I--Delikates, Cortland, James Grieve and Lired, II--Jonathan, Golden Delicious, Jonagold and Idared. Genetic similarity between Delikates, Cortland and James Grieve was 68.6, 70.8%, respectively and between cultivar Jonagold, Jonathan and Golden Delicous was 79.8, 85.2%, respectively.

摘要

该研究的目的是确定来自西波美拉尼亚理工大学什切青分校拉伊科沃水果种植研究站收集的八个苹果品种的遗传变异性

Delikates、科特兰、詹姆斯·格里夫、利雷、乔纳森、金冠、乔纳金和艾达雷德。Delikates品种是由科特兰和詹姆斯·格里夫两个品种杂交获得的，而利雷品种是詹姆斯·格里夫的一个芽变品种。第二个品种——乔纳金是由乔纳森和金冠杂交获得的。艾达雷德品种是乔纳森和瓦格纳杂交获得的杂种。在40个引物中，最终选择了17个用于研究。这些引物共扩增出183个位点（872个扩增子），其中34个（18.5%）是单态的，128个（69.5%）是多态的，22个（12%）是品种特异性的。每个苹果品种都检测到了特异性的ISSR产物。使用UPGMA方法构建了一个聚类图，该图显示出两个不同的聚类：I——Delikates、科特兰、詹姆斯·格里夫和利雷，II——乔纳森、金冠、乔纳金和艾达雷德。Delikates、科特兰和詹姆斯·格里夫之间的遗传相似性分别为68.6%、70.8%，乔纳金、乔纳森和金冠品种之间的遗传相似性分别为79.8%、85.2%。