一个含有昆虫特异性毒素 BmK IT1 的重组 AeDNA 在白纹伊蚊上表现出了增强的致病性。
A recombinant AeDNA containing the insect-specific toxin, BmK IT1, displayed an increasing pathogenicity on Aedes albopictus.
机构信息
School of Public Health and Tropical Medicine, Southern Medical University, Guangdong, People's Republic of China.
出版信息
Am J Trop Med Hyg. 2010 Sep;83(3):614-23. doi: 10.4269/ajtmh.2010.10-0074.
The Aedes aegypti densovirus (AeDNV) has previously shown potential in mosquito control. To improve its efficacy as a biopesticide, the gene for an excitatory insect-specific toxin from Buthus martensii Karsch (BmK IT1) was inserted into the AeDNV genome and cloned into pUCA plasmid. The coding sequence for green fluorescent protein was ligated to the C-terminus of the BmK IT1 gene as a screening marker. Recombinant and helper plasmids were cotransfected into C6/36 cells; wild-type viruses were the controls. The recombinant viruses were identified and quantified by real-time polymerase chain reaction and exposed to Ae. albopictus larvae for the evaluation of its bioinsecticidal activity. LT(50) and LD(50) bioassays showed that the recombinant AeDNV had stronger and faster pathogenic effects on Ae. albopictus than the wild-type virus. This is the first report on the recombinant AeDNA containing the insect-specific toxin, BmK IT1, which may be used to develop a novel type of insecticide.
埃及伊蚊浓核病毒（AeDNV）在蚊虫控制方面具有很大的潜力。为了提高其作为生物农药的功效，将来自蝎子的一种兴奋性昆虫特异性毒素基因（BmK IT1）插入 AeDNV 基因组并克隆到 pUCA 质粒中。绿色荧光蛋白的编码序列被连接到 BmK IT1 基因的 C 末端作为筛选标记。重组和辅助质粒被共转染到 C6/36 细胞中；野生型病毒作为对照。通过实时聚合酶链反应鉴定和定量重组病毒，并将其暴露于白纹伊蚊幼虫中以评估其生物杀虫活性。LT（50）和 LD（50）生物测定表明，重组 AeDNV 对白纹伊蚊的致病效果比野生型病毒更强、更快。这是首例关于含有昆虫特异性毒素 BmK IT1 的重组 AeDNA 的报告，可能用于开发新型杀虫剂。
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