去甲斑蝥素抑制癌细胞中的DNA复制起始蛋白Cdc6
[Norcantharidin inhibits DNA replication initiation protein Cdc6 in cancer cells].
作者信息
Li Jin-long, Cai Yu-chen, Hu Zhi-ming, Gao Ji-min
机构信息
Institute of Biotherapy, School of Biotechnology, Southern Medical University, Guangzhou 510515, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2010 Aug;30(8):1851-3.
OBJECTIVE
To explore the inhibitory effect of norcantharidin (NCTD) on the expression of DNA replication initiation protein Cdc6 in cancer cells.
METHODS
MTT assay was performed to detect the inhibitory effect on different cancer cell lines, including HeLa, HepG2, Jurkat and Ramos cells. The effect of NCTD on Cdc6 protein level was detected by Western blotting, and BrdU incorporation assay was used to evaluate the DNA replication of the cells.
RESULTS
NCTD significantly inhibited the proliferation of the cells and caused degradation of Cdc6 protein to result in the inhibition of the DNA replication of the cells shown by BrdU incorporation assay.
CONCLUSION
NCTD can induce the degradation of Cdc6 in cancer cells to produce an anti-cancer effect.
目的
探讨去甲斑蝥素（NCTD）对癌细胞中DNA复制起始蛋白Cdc6表达的抑制作用。
方法
采用MTT法检测NCTD对不同癌细胞系（包括HeLa、HepG2、Jurkat和Ramos细胞）的抑制作用。通过蛋白质免疫印迹法检测NCTD对Cdc6蛋白水平的影响，并用BrdU掺入法评估细胞的DNA复制情况。
结果
NCTD显著抑制细胞增殖，并导致Cdc6蛋白降解，从而抑制细胞的DNA复制，这在BrdU掺入法中得到证实。
结论
NCTD可诱导癌细胞中Cdc6的降解，从而产生抗癌作用。
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