通过频域荧光法观察到的端到端扩散对分子内能量转移的影响。

Influence of end-to-end diffusion on intramolecular energy transfer as observed by frequency-domain fluorometry.

作者信息

Lakowicz J R, Wiczk W, Gryczynski I, Szmacinski H, Johnson M L

机构信息

University of Maryland, School of Medicine, Department of Biological Chemistry, Baltimore 21201.

出版信息

Biophys Chem. 1990 Oct;38(1-2):99-109. doi: 10.1016/0301-4622(90)80044-8.

DOI:10.1016/0301-4622(90)80044-8

PMID:2085654

Abstract

We investigated the influence of end-to-end diffusion on intramolecular energy transfer between a naphthalene donor and dansyl acceptor linked by polymethylene chain. A range of viscosities from 0.6 to 200 cP were obtained using propylene glycol at different temperatures (0-80 degrees C) and methanol at 20 degrees C. The intensity decays of naphthalene were measured in the frequency domain. Several theoretical models, including distance distributions, were used to fit the data. The results indicate that end-to-end diffusion of flexible donor-acceptor pairs can be detected and quantified using frequency-domain fluorometry, even in the presence of a distribution of donor-to-acceptor distances.

摘要

我们研究了端到端扩散对由聚亚甲基链连接的萘供体和丹磺酰受体之间分子内能量转移的影响。通过在不同温度（0 - 80摄氏度）下使用丙二醇以及在20摄氏度下使用甲醇，获得了一系列粘度范围为0.6至200厘泊的溶液。在频域中测量了萘的强度衰减。使用了包括距离分布在内的几种理论模型来拟合数据。结果表明，即使存在供体到受体距离的分布，使用频域荧光法也可以检测和量化柔性供体 - 受体对的端到端扩散。