运用荧光原位杂交技术（FISH）快速鉴定血培养中的病原体。

Rapid identification of pathogens in blood culture with fluorescent in situ hybridization (FISH).

作者信息

Horváth Andrea, Kristóf Katalin, Konkoly-Thege Marianne, Nagy K

机构信息

Semmelweis University, Institute of Medical Microbiology, and Central Microbiology Laboratory, Szent István Hospital, Budapest, Hungary.

出版信息

Acta Microbiol Immunol Hung. 2010 Sep;57(3):225-34. doi: 10.1556/AMicr.57.2010.3.7.

DOI:10.1556/AMicr.57.2010.3.7

PMID:20870594

Abstract

Rapid identification of pathogens in bloodstream infections is of utmost importance to improve survival of septic patients. Fluorescent in situ hybridization (FISH) accelerates the identification of most frequent bacterial and yeast pathogens of sepsis. In this study, 210 positive blood cultures were tested with FISH method and the results were evaluated comparing to the traditional cultivation based results. Overall agreement between FISH and conventional identification was 91.4%, with better results for Gram-negative bacteria than for Gram-positives (100% and 89.5%, respectively). FISH results were obtained within 1 hour. FISH may serve as a useful tool to supplement traditional microbiological methods for rapid, provisional identification of sepsis pathogens.

摘要

快速鉴定血流感染中的病原体对于提高脓毒症患者的生存率至关重要。荧光原位杂交（FISH）技术可加速对脓毒症中最常见的细菌和酵母病原体的鉴定。在本研究中，采用FISH方法对210份阳性血培养物进行检测，并将结果与基于传统培养的结果进行比较评估。FISH与传统鉴定方法的总体一致性为91.4%，革兰氏阴性菌的结果优于革兰氏阳性菌（分别为100%和89.5%）。FISH结果可在1小时内获得。FISH可作为一种有用的工具，补充传统微生物学方法，用于快速初步鉴定脓毒症病原体。