Wang Xin, Zhao Chunyuan, Liu Ming
Department of Otolaryngology-Head and Neck Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin,150086, China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2010 Jul;24(14):652-4.
To detect the expression and distribution of surfactant A (SP-A) in nasal polyps and to probe into its significance in the pathology of nasal polyps.
Immunohistochemical staining and RT-PCR (reverse transcription polymerase chain reaction) were explored to detect SP-A in nasal polyps and controls.
In nasal polyp tissues, SP-A expressed not only in the cytoplasm of the epithelium but also in the cytoplasm of the plasma cells. Moreover it expressed in the serous glands but not in the mucous glands. The expression of SP-A was distributed in the same location of turbinates. But the expression of SP-A between nasal polyps and turbinates differed significantly (P < 0.05). SP-A mRNA was detected in the nasal polyps and controls. The expression potency ratio of SP-A/beta-actin in nasal polyps was stronger than in turbinates (P < 0.05).
Both nasal polyps and nasal mucosa expressed SP-A mRNA and protein, but the expression was stronger in nasal polyps. The role of SP-A in the innate immunity may contribute to the pathogenesis of nasal polyps. SP-A may become the new target in the therapy of chronic rhinosinusitis.
检测表面活性物质A（SP-A）在鼻息肉中的表达及分布情况，并探讨其在鼻息肉病理过程中的意义。
采用免疫组织化学染色及逆转录聚合酶链反应（RT-PCR）检测鼻息肉组织及对照组织中的SP-A。
在鼻息肉组织中，SP-A不仅在上皮细胞的胞质中表达，还在浆细胞的胞质中表达。此外，它在浆液性腺中表达，而在黏液性腺中不表达。SP-A的表达分布于鼻甲的相同部位。但鼻息肉与鼻甲之间SP-A的表达差异有统计学意义（P < 0.05）。在鼻息肉组织及对照组织中均检测到SP-A mRNA。鼻息肉中SP-A/β-肌动蛋白的表达强度比值高于鼻甲（P < 0.05）。
鼻息肉组织和鼻黏膜均表达SP-A mRNA和蛋白，但在鼻息肉中的表达更强。SP-A在固有免疫中的作用可能与鼻息肉的发病机制有关。SP-A可能成为慢性鼻-鼻窦炎治疗的新靶点。
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