三种不同海洋蓝藻培养物藻红蛋白的纯化、特性分析与比较。

Purification, characterization and comparison of phycoerythrins from three different marine cyanobacterial cultures.

机构信息

BRD School of Biosciences, Sardar Patel Maidan, Vadtal Road, Satellite Campus, Post Box No. 39, Sardar Patel University, Vallabh Vidyanagar 388 120, Anand, Gujarat, India.

出版信息

Bioresour Technol. 2011 Jan;102(2):1795-802. doi: 10.1016/j.biortech.2010.09.025. Epub 2010 Sep 21.

DOI:10.1016/j.biortech.2010.09.025

PMID:20889334

Abstract

The present study is focused on purification, characterization and comparison of phycoerythrins from three different marine cyanobacterial cultures--hormidium sp. A27 DM, Lyngbya sp. A09 DM and Halomicronema sp. A32 DM. 'Phycoerythrin' was successfully purified and characterized. On SDS-PAGE, the PE purified from all three young cultures showed four bands--corresponding to α and β subunits of each of PE-I and PE-II. However, phycoerythrin purified after prolonged growth of Phormidium sp. A27 DM and Halomicronema sp. A32DM showed only one band corresponding to 14 kDa whereas Lyngbya sp. A09 DM continued to produce uncleaved phycoerythrin. The absorption spectra of purified PEs from all the three young and old cultures showed variations however the fluorescence studies of the purified PEs in all cases gave the emission spectra at around 580 nm. The described work is of great importance to understand the role of phycoerythrin in adapting cyanobacteria to stress conditions.

摘要

本研究专注于从三种不同的海洋蓝细菌培养物——Hormidium sp. A27 DM、Lyngbya sp. A09 DM 和 Halomicronema sp. A32 DM 中纯化、表征和比较藻红蛋白。藻红蛋白成功地被纯化和表征。在 SDS-PAGE 上，来自所有三种年轻培养物的纯化 PE 显示出四条带——分别对应于 PE-I 和 PE-II 的每个α和β亚基。然而，经过 Phormidium sp. A27 DM 和 Halomicronema sp. A32DM 的长时间生长后纯化的藻红蛋白仅显示出一条对应于 14 kDa 的带，而 Lyngbya sp. A09 DM 继续产生未切割的藻红蛋白。所有年轻和年老培养物的纯化 PE 的吸收光谱显示出变化，然而，在所有情况下，对纯化 PE 的荧光研究都给出了约 580nm 的发射光谱。这项描述性的工作对于理解藻红蛋白在使蓝细菌适应应激条件中的作用具有重要意义。