OLETF 大鼠 Thy-1.1 肾炎加重糖尿病肾病。

Aggravation of diabetic nephropathy in OLETF rats by Thy-1.1 nephritis.

机构信息

Division of Nephrology and Rheumatology, Department of Internal Medicine, Faculty of Medicine, Fukuoka University, 7-45-1 Nanakuma, Jonann-ku, Fukuoka 814-0180, Japan.

出版信息

Clin Exp Nephrol. 2011 Feb;15(1):25-9. doi: 10.1007/s10157-010-0349-1. Epub 2010 Oct 5.

DOI:10.1007/s10157-010-0349-1

PMID:20922555

Abstract

OBJECTIVE

To clarify whether the induction of Thy-1.1 nephritis aggravates diabetic nephropathy in the Otsuka Long-Evans Tokushima Fatty (OLETF) rat, which is a model of diabetes mellitus.

METHOD

Forty-week-old OLETF rats were divided into 2 groups according to treatment: (1) 1 mg/kg body weight of OX7, an anti-Thy1.1 antibody (administered intravenously) (Group T, n = 14); (2) 0.9% saline (Group C, n = 14). The histological findings for the kidneys and the index of glomerulosclerosis (IGS) were determined 20 weeks after administration, and urine and serum chemistry were also assessed. The same procedure was performed as a control in 2 groups of Long-Evans Tokushima Otsuka (LETO) rats (i.e., nondiabetic OLETF rats).

RESULTS

The urinary protein excretion values and the levels of serum albumin in the OX7-treated OLETF rats were significantly higher and lower than those in the untreated OLETF rats, respectively. Total cholesterol was significantly increased in the OX7-treated OLETF rats compared with the untreated OLETF rats. In the histological analysis, IGS was significantly higher in the OX7-treated OLETF rats than in the untreated OLETF rats. Neither deteriorations in the laboratory assessment values nor histological alterations were seen in the LETO rats.

CONCLUSION

These findings indicate that an anti-Thy-1.1 antibody irreversibly aggravates diabetic nephropathy in the OLETF rat.

摘要

目的

阐明诱导 Thy-1.1 肾炎是否会加重 Otsuka Long-Evans Tokushima Fatty（OLETF）大鼠的糖尿病肾病，OLETF 大鼠是糖尿病的模型。

方法

将 40 周龄的 OLETF 大鼠按治疗分为 2 组：（1）OX7，一种抗 Thy1.1 抗体（静脉注射），1mg/kg 体重（组 T，n=14）；（2）0.9%生理盐水（组 C，n=14）。给药 20 周后测定肾脏组织学发现和肾小球硬化指数（IGS），并评估尿液和血清化学。在两组 Long-Evans Tokushima Otsuka（LETO）大鼠（即非糖尿病 OLETF 大鼠）中进行了相同的程序作为对照。

结果

OX7 治疗的 OLETF 大鼠的尿蛋白排泄值和血清白蛋白水平明显高于未治疗的 OLETF 大鼠，分别。OX7 治疗的 OLETF 大鼠的总胆固醇明显高于未治疗的 OLETF 大鼠。在组织学分析中，OX7 治疗的 OLETF 大鼠的 IGS 明显高于未治疗的 OLETF 大鼠。LETO 大鼠的实验室评估值和组织学改变均未见恶化。