聚肌胞苷酸诱导气道平滑肌细胞中TLR3及白细胞介素-8、嗜酸性粒细胞趋化因子的表达
[Expression of TLR3 and IL-8, Eotaxin in the airway smooth muscle cells induced by PolyI:C].
作者信息
Yan Ming-Hua, Zhu Shu-Yang, Duan Cun-Ling, Ji Gui-Juan
机构信息
Department of Respiratory Medicine, the Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2010 Oct;26(10):966-8, 972.
AIM
to observe the effect of PolyI:C on the expression of TLR3 and inflammation cytokine in the airway smooth muscle cells ASMCs of rat, to investigate relationship between the expression of Toll-like receptor 3 (TLR3) in the airway smooth muscle cells and airway inflammation.
METHODS
airway smooth muscle cells were derived from rat airway tissue and were cultured in vitro, ASMCs were divided into 2 groups: control group and PolyI:C group, PolyI:C group was divided into the different time spots. The mRNA expression of TLR3 was measured by RT-PCR. The level of NF-kappaB was measured by Western blot. The concertration of IL-8 and Eotaxin in ASMCs supernatant were determined by ELISA.
RESULTS
the expression of TLR3 mRNA and NF-kappaB protein in the ASMCs in PolyI:C group was significantly higher than those in control group(P<0.05), the concentration of IL-8 and Eotaxin in the ASMCs in PolyI:C group was significantly higher compared to control group (P<0.05); and have time dependent manner.
CONCLUSION
poly I:C promoted the expression of TLR3 in ASMCs, activated NF-kappaB, incresead the concentration of IL-8 and Eotaxin, involved in airway inflammation of asthma.
目的
观察聚肌胞苷酸（PolyI:C）对大鼠气道平滑肌细胞（ASMCs）中Toll样受体3（TLR3）表达及炎症细胞因子的影响，探讨气道平滑肌细胞中Toll样受体3（TLR3）表达与气道炎症之间的关系。
方法
从大鼠气道组织分离气道平滑肌细胞并进行体外培养，将ASMCs分为2组：对照组和PolyI:C组，PolyI:C组再分为不同时间点。采用逆转录聚合酶链反应（RT-PCR）检测TLR3的mRNA表达。采用蛋白质免疫印迹法检测核因子κB（NF-κB）水平。采用酶联免疫吸附测定（ELISA）法测定ASMCs上清液中白细胞介素8（IL-8）和嗜酸性粒细胞趋化因子的浓度。
结果
PolyI:C组ASMCs中TLR3 mRNA和NF-κB蛋白表达均显著高于对照组（P<0.05），PolyI:C组ASMCs中IL-8和嗜酸性粒细胞趋化因子浓度显著高于对照组（P<0.05）；且呈时间依赖性。
结论
聚肌胞苷酸（PolyI:C）促进ASMCs中TLR3表达，激活NF-κB，增加IL-8和嗜酸性粒细胞趋化因子浓度，参与哮喘气道炎症。
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