Shaw Peter J
Department of Cell and Developmental Biology, John Innes Centre Colney Lane, Colney, Norwich, NR4 7UH UK.
F1000 Biol Rep. 2010 Mar 15;2:18. doi: 10.3410/B2-18.
Chromatin conformation capture (3C) and related techniques have become well-established methods to examine which distal DNA sequences are spatially located close to a locus of interest. Hi-C is a new addition to the growing set of 3C-based techniques that has extended the approach to a genome-wide analysis of nuclear chromatin using high-throughput DNA sequencing to generate global interaction maps for the entire genome.
染色质构象捕获技术(3C)及相关技术已成为成熟的方法,用于检测哪些远端DNA序列在空间上靠近感兴趣的基因座。Hi-C是基于3C技术不断发展的新成员,它通过高通量DNA测序将该方法扩展到全基因组范围的核染色质分析,以生成整个基因组的全局相互作用图谱。