从淋巴器官分离的 T 细胞和用刀豆蛋白 A 培养的脾细胞中的鸡趋化因子受体。

Chicken chemokine receptors in T cells isolated from lymphoid organs and in splenocytes cultured with concanavalin A.

机构信息

Department of Animal Sciences, Ohio Agricultural Research and Development Center, Wooster 44691, USA.

出版信息

Poult Sci. 2010 Nov;89(11):2419-25. doi: 10.3382/ps.2010-00968.

DOI:10.3382/ps.2010-00968

PMID:20952705

Abstract

Chemokine receptors guide immune cells to specific organs during health and disease. The mRNA content of the chemokine receptors CCR2, CCR4, CCR5, CCR6, CCR7, CCR8, CCR9, CXCR4, CXCR5, and CX3CR1 in CD4(+) cells (T-helper cells) isolated from blood, bursa, cecal tonsil, spleen, and thymus and in CD8(+) cells (T-cytotoxic cells) isolated from blood, cecal tonsil, spleen, and thymus were investigated. The CD4(+) cells isolated from thymus had the highest amount of CCR7 and CCR8 mRNA. The CD4(+) cells isolated from bursa, cecal tonsil, and thymus had the highest amount of CCR5 mRNA. The CD4(+) cells isolated from cecal tonsils had the highest amount of CCR9 mRNA. The CD4(+) cells isolated from bursa and thymus had the highest amount of CXCR5 mRNA. The CD8(+) cells isolated from cecal tonsil had the highest mRNA amount of all receptors studied except CCR9 and CX3CR1. The CD4(+) cells treated with concanavalin A had increased CCR2, CCR4, CCR7, CCR8, and CXCR5 mRNA amounts at 24 h of stimulation. The CD8(+) cells treated with concanavalin A had increased CCR4 mRNA at 72 h, increased CCR6 mRNA at 24 h, and decreased CCR8 and CXCR4 mRNA at 24 h of stimulation.

摘要

趋化因子受体在健康和疾病期间引导免疫细胞到达特定器官。从血液、囊、盲肠扁桃体、脾脏和胸腺中分离的 CD4(+)细胞（辅助性 T 细胞）和从血液、盲肠扁桃体、脾脏和胸腺中分离的 CD8(+)细胞（细胞毒性 T 细胞）中的趋化因子受体 CCR2、CCR4、CCR5、CCR6、CCR7、CCR8、CCR9、CXCR4、CXCR5 和 CX3CR1 的 mRNA 含量进行了研究。从胸腺中分离的 CD4(+)细胞具有最高量的 CCR7 和 CCR8 mRNA。从囊、盲肠扁桃体和胸腺中分离的 CD4(+)细胞具有最高量的 CCR5 mRNA。从盲肠扁桃体中分离的 CD4(+)细胞具有最高量的 CCR9 mRNA。从盲肠和胸腺中分离的 CD4(+)细胞具有最高量的 CXCR5 mRNA。除 CCR9 和 CX3CR1 外，从盲肠扁桃体中分离的 CD8(+)细胞具有所有研究受体中最高的 mRNA 量。用刀豆蛋白 A 处理的 CD4(+)细胞在刺激 24 小时时增加 CCR2、CCR4、CCR7、CCR8 和 CXCR5 mRNA 量。用刀豆蛋白 A 处理的 CD8(+)细胞在 72 小时时增加 CCR4 mRNA，在 24 小时时增加 CCR6 mRNA，在 24 小时时减少 CCR8 和 CXCR4 mRNA。