大脑中的表观遗传变化：测量整体组蛋白修饰
Epigenetic changes in the brain: measuring global histone modifications.
作者信息
Rumbaugh Gavin, Miller Courtney A
机构信息
Department of Neuroscience, The Scripps Research Institute Florida, Jupiter, FL, USA.
出版信息
Methods Mol Biol. 2011;670:263-74. doi: 10.1007/978-1-60761-744-0_18.
Abstract
Recent years have witnessed an explosion of research on the role of epigenetic modifications, such as DNA methylation and histone protein acetylation and phosphorylation, in neuroscience. These changes exert control over gene expression and have been shown to play important roles in a variety of neural processes, including learning and memory. We and others have also recently shown that epigenetic changes may contribute to neurodegenerative disorders, such as Alzheimer's disease. Western blot analysis with antibodies raised against specific histone modifications is a relatively simple technique able to reveal the type, location, and degree of histone posttranslational modifications produced by an experimental manipulation. Here we provide a step-by-step protocol for isolating histone proteins from tissue and measuring these posttranslational modifications.
摘要
近年来，关于表观遗传修饰（如DNA甲基化、组蛋白乙酰化和磷酸化）在神经科学中的作用的研究呈爆发式增长。这些变化控制着基因表达，并已被证明在包括学习和记忆在内的各种神经过程中发挥重要作用。我们和其他人最近还表明，表观遗传变化可能导致神经退行性疾病，如阿尔茨海默病。使用针对特定组蛋白修饰产生的抗体进行蛋白质免疫印迹分析是一种相对简单的技术，能够揭示实验操作产生的组蛋白翻译后修饰的类型、位置和程度。在这里，我们提供了一个从组织中分离组蛋白并测量这些翻译后修饰的分步方案。
相似文献
1
Epigenetic changes in the brain: measuring global histone modifications.
Methods Mol Biol. 2011;670:263-74. doi: 10.1007/978-1-60761-744-0_18.
2
DNA methylation and histone post-translational modification stability in post-mortem brain tissue.
Clin Epigenetics. 2019 Jan 11;11(1):5. doi: 10.1186/s13148-018-0596-7.
3
Insights into the Role of Histone Methylation in Brain Aging and Potential Therapeutic Interventions.
Int J Mol Sci. 2023 Dec 11;24(24):17339. doi: 10.3390/ijms242417339.
4
Histone Posttranslational Modifications in Schizophrenia.
Adv Exp Med Biol. 2017;978:237-254. doi: 10.1007/978-3-319-53889-1_13.
5
Extraction, Identification, and Quantification of Histones from Small Quantities of Specific Brain Tissue.
Curr Protoc Neurosci. 2016 Jul 1;76:4.38.1-4.38.20. doi: 10.1002/cpns.11.
6
The role of global histone post-translational modifications during mammalian hibernation.
Cryobiology. 2017 Apr;75:28-36. doi: 10.1016/j.cryobiol.2017.02.008. Epub 2017 Feb 28.
7
Epigenomics in stress tolerance of plants under the climate change.
Mol Biol Rep. 2023 Jul;50(7):6201-6216. doi: 10.1007/s11033-023-08539-6. Epub 2023 Jun 9.
8
The pattern of histone H3 epigenetic posttranslational modifications is regulated by the VRK1 chromatin kinase.
Epigenetics Chromatin. 2023 May 13;16(1):18. doi: 10.1186/s13072-023-00494-7.
9
Histone Modifications in Aging: The Underlying Mechanisms and Implications.
Curr Stem Cell Res Ther. 2018;13(2):125-135. doi: 10.2174/1574888X12666170817141921.
10
Epigenetic regulation and chromatin remodeling in learning and memory.
Exp Mol Med. 2017 Jan 13;49(1):e281. doi: 10.1038/emm.2016.140.
引用本文的文献
1
Therapeutic Effects of Engineered Exosomes from RAW264.7 Cells Overexpressing hsa-let-7i-5p against Sepsis in Mice-A Comparative Study with Human Placenta-Derived Mesenchymal Stem Cell Exosomes.
J Pers Med. 2024 Jun 9;14(6):619. doi: 10.3390/jpm14060619.
2
Neuroinflammaging: A Tight Line Between Normal Aging and Age-Related Neurodegenerative Disorders.
Aging Dis. 2024 Aug 1;15(4):1726-1747. doi: 10.14336/AD.2023.1001.
3
Exploring Potential Epigenetic Biomarkers for Colorectal Cancer Metastasis.
Int J Mol Sci. 2024 Jan 10;25(2):874. doi: 10.3390/ijms25020874.
4
Histone Extraction from Human Articular Cartilage for the Study of Epigenetic Regulation in Osteoarthritis.
Int J Mol Sci. 2022 Mar 20;23(6):3355. doi: 10.3390/ijms23063355.
5
Small-molecule Akt-activation in airway cells induces NO production and reduces IL-8 transcription through Nrf-2.
Respir Res. 2021 Oct 19;22(1):267. doi: 10.1186/s12931-021-01865-y.
6
Low folate induces abnormal neuronal maturation and DNA hypomethylation of neuronal differentiation-related genes in cultured mouse neural stem and progenitor cells.
Heliyon. 2021 Sep 24;7(9):e08071. doi: 10.1016/j.heliyon.2021.e08071. eCollection 2021 Sep.
7
Epigenetic Regulation of Circadian Clocks and Its Involvement in Drug Addiction.
Genes (Basel). 2021 Aug 19;12(8):1263. doi: 10.3390/genes12081263.
8
Neonatal Alcohol Exposure in Mice Induces Select Differentiation- and Apoptosis-Related Chromatin Changes Both Independent of and Dependent on Sex.
Front Genet. 2020 Feb 11;11:35. doi: 10.3389/fgene.2020.00035. eCollection 2020.
9
Inhibition of the G9a/GLP histone methyltransferase complex modulates anxiety-related behavior in mice.
Acta Pharmacol Sin. 2018 May;39(5):866-874. doi: 10.1038/aps.2017.190. Epub 2018 Feb 8.
10
Aging alters mRNA expression of amyloid transporter genes at the blood-brain barrier.
Neurobiol Aging. 2017 Sep;57:178-185. doi: 10.1016/j.neurobiolaging.2017.05.011. Epub 2017 May 19.
本文引用的文献
1
Inhibitors of class 1 histone deacetylases reverse contextual memory deficits in a mouse model of Alzheimer's disease.
Neuropsychopharmacology. 2010 Mar;35(4):870-80. doi: 10.1038/npp.2009.197. Epub 2009 Dec 9.
2
Protein phosphatase 1 regulates the histone code for long-term memory.
J Neurosci. 2009 Oct 14;29(41):13079-89. doi: 10.1523/JNEUROSCI.3610-09.2009.
3
Epigenetic codes in cognition and behaviour.
Behav Brain Res. 2008 Sep 1;192(1):70-87. doi: 10.1016/j.bbr.2008.01.021. Epub 2008 Feb 12.
4
The histone deacetylase inhibitor valproic acid enhances acquisition, extinction, and reconsolidation of conditioned fear.
Learn Mem. 2008 Jan 3;15(1):39-45. doi: 10.1101/lm.801108. Print 2008 Jan.
5
DNA methylation and histone acetylation work in concert to regulate memory formation and synaptic plasticity.
Neurobiol Learn Mem. 2008 May;89(4):599-603. doi: 10.1016/j.nlm.2007.07.016. Epub 2007 Sep 18.
6
Histone deacetylase inhibitors enhance memory and synaptic plasticity via CREB:CBP-dependent transcriptional activation.
J Neurosci. 2007 Jun 6;27(23):6128-40. doi: 10.1523/JNEUROSCI.0296-07.2007.
7
Transgenic mice expressing a truncated form of CREB-binding protein (CBP) exhibit deficits in hippocampal synaptic plasticity and memory storage.
Learn Mem. 2005 Mar-Apr;12(2):111-9. doi: 10.1101/lm.86605.
8
Regulation of histone acetylation during memory formation in the hippocampus.
J Biol Chem. 2004 Sep 24;279(39):40545-59. doi: 10.1074/jbc.M402229200. Epub 2004 Jul 23.
9
Increased histone acetyltransferase and lysine acetyltransferase activity and biphasic activation of the ERK/RSK cascade in insular cortex during novel taste learning.
J Neurosci. 2001 May 15;21(10):3383-91. doi: 10.1523/JNEUROSCI.21-10-03383.2001.
10
Role of covalent modifications of histones in regulating gene expression.
Gene. 1999 Nov 15;240(1):1-12. doi: 10.1016/s0378-1119(99)00405-9.
Zotero 插件