微泡和超声对微循环的影响：对金黄地鼠颊囊的观察。

Effects of microbubbles and ultrasound on the microcirculation: observation on the hamster cheek pouch.

机构信息

Laboratory for Clinical and Experimental Research on Vascular Biology, Biomedical Center, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.

出版信息

J Am Soc Echocardiogr. 2010 Dec;23(12):1323-30. doi: 10.1016/j.echo.2010.09.006. Epub 2010 Oct 23.

DOI:10.1016/j.echo.2010.09.006

PMID:20970959

Abstract

BACKGROUND

In view of the debate on the safety of using ultrasound associated with microbubbles, we investigated the microvascular effects in situations of potential clinical interest.

METHODS

Ultrasound, microbubbles, and their association were evaluated on the cheek pouch microcirculation in 96 male hamsters (n = 6 in each group): control; ischemia-reperfusion, 30 minutes total ischemia followed by reperfusion; hyperinsulinemia and hyperglycemia (substitution of the drinking water by 10% fructose solution for 3 months); and endotoxic shock (induced by Escherichia coli lipopolysaccharide). The heart rate, mean arterial pressure, number of rolling and adhered leukocytes, and arteriolar and venular diameters were analyzed.

RESULTS

Heart rate and mean arterial pressure were lower in the endotoxic shock group than in the control group. Ultrasound alone or associated with microbubbles decreased the number of rolling and adhered leukocytes in the ischemia-reperfusion and hyperinsulinemia and hyperglycemia groups and increased venular diameter in the ischemia-reperfusion group.

CONCLUSION

The use of ultrasound decreases inflammation. Although it has not been established that pseudo-anaphylaxis can be assessed by intravital microscopy, microbubble administration does not increase venular leukocyte adhesion.

摘要

背景

鉴于有关超声联合微泡应用安全性的争论，我们研究了在具有潜在临床意义的情况下微血管的效应。

方法

我们评估了超声、微泡及其联合应用对 96 只雄性仓鼠颊囊微循环的影响（每组 6 只）：对照组；缺血再灌注组，总缺血 30 分钟后再灌注；高胰岛素血症和高血糖组（将饮用水替换为 10%果糖溶液 3 个月）；内毒素休克组（大肠杆菌脂多糖诱导）。分析心率、平均动脉压、滚动和黏附白细胞的数量以及微动脉和微静脉的直径。