Department of Applied Mechanics (Biotechnology), Motilal Nehru National Institute of Technology, Allahabad 211004, India.
Anal Chim Acta. 2010 Dec 3;682(1-2):66-71. doi: 10.1016/j.aca.2010.09.041. Epub 2010 Oct 21.
A rapid and semi-quantitative immunochromatographic strip (ICS) test for cortisol analysis in serum was developed. The test strip was based on a competitive assay format. Colloidal gold nanoparticles were synthesized and coupled with cortisol-3-carboxymethyloxime-adipic acid dihydrazide-bovine serum albumin (F-3-CMO-ADH-BSA) antigen to directly compete with cortisol in human serum samples. F-3-CMO-ADH-BSA-gold label and uncoupled colloidal gold nanoparticles were appropriately characterized using UV-vis spectroscopy, transmission electron microscopy and atomic force microscopy. Anticortisol antibody raised against F-3-CMO-BSA immunogen in New Zealand white rabbits was coated on the NC membrane as test line. Anti-BSA antibody was used as control line. The lower detection limit of the ICS test was 30 ngmL(-1) with visual detection and was completed in 10 min. About 30 human serum samples were also analyzed by the developed strip test and their range of cortisol concentration was established. The developed ICS test is rapid, economic and user friendly.
一种用于血清中皮质醇分析的快速半定量免疫层析试纸条（ICS）被开发出来。该试纸条基于竞争测定法。合成了胶体金纳米颗粒，并将其与皮质醇-3-羧甲基肟-己二酰二肼-牛血清白蛋白（F-3-CMO-ADH-BSA）抗原偶联，以直接与人体血清样本中的皮质醇竞争。F-3-CMO-ADH-BSA-金标记和未偶联的胶体金纳米颗粒使用紫外-可见分光光度法、透射电子显微镜和原子力显微镜进行了适当的表征。用新西兰白兔针对 F-3-CMO-BSA 免疫原产生的抗皮质醇抗体作为测试线被涂覆在 NC 膜上。抗 BSA 抗体被用作控制线。该 ICS 测试的最低检测限为 30ngmL(-1)，具有目视检测功能，且在 10 分钟内完成。还使用开发的条带测试分析了约 30 个人体血清样本，并确定了它们的皮质醇浓度范围。开发的 ICS 测试快速、经济且易于使用。
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