用于规范生物分析的自动化样品制备：一种使用机器人液体处理的集成多重分析物提取平台。
Automated sample preparation for regulated bioanalysis: an integrated multiple assay extraction platform using robotic liquid handling.
作者信息
Tweed Joseph A, Gu Zhenhua, Xu Hui, Zhang Guodong, Nouri Parya, Li Ming, Steenwyk Rick
机构信息
Pharmacokinetics, Dynamics & Metabolism, Pfizer Global Research and Development, Pfizer Inc., Groton, CT 06340, USA.
出版信息
Bioanalysis. 2010 Jun;2(6):1023-40. doi: 10.4155/bio.10.55.
BACKGROUND
A novel approach for regulated bioanalytical sample preparation has been developed to combine multiple types of extraction techniques into one integrated and automated sample-preparation suite that pairs a graphical user interface with the Hamilton Microlab(®) STAR robotic liquid handler.
RESULTS
The multi-assay sample-preparation suite is composed of three bioanalytical extraction techniques: protein precipitation, solid-phase extraction and liquid-liquid extraction. Validation data provided highly reproducible and robust results for each respective automated extraction technique.
CONCLUSION
The user-friendly graphical user interface and modular method design provide a flexible and versatile approach for routine bioanalytical sample-preparation and is the first fully integrated multiple assay sample-preparation suite for regulated bioanalysis.
背景
已开发出一种用于规范生物分析样品制备的新方法，可将多种提取技术整合到一个集成化、自动化的样品制备套件中，该套件将图形用户界面与Hamilton Microlab(®) STAR机器人液体处理仪相结合。
结果
多分析物样品制备套件由三种生物分析提取技术组成：蛋白质沉淀、固相萃取和液液萃取。验证数据为每种自动化提取技术提供了高度可重复且可靠的结果。
结论
用户友好的图形用户界面和模块化方法设计为常规生物分析样品制备提供了一种灵活通用的方法，并且是首个用于规范生物分析的完全集成的多分析物样品制备套件。
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