Wang Hao, Yu Guang-li, Zhao Xia, Hao Cui, Li Guang-sheng, Wang Pei-pei
Key Laboratory of Glycoscience and Glycoengineering of Shandong Province, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2010 Sep;30(9):2484-7.
Glycosaminoglycans (GAGs) were extracted and purified from Wistar rat kidneys by two-step enzymatic hydrolysis and ion exchange chromatography. The sorts of GAGs were identified by electrophoresis on cellulose acetate membrane and separated on a weak anion-exchange column. Those purified GAGs were further totally hydrolyzed with specific glycosaminoglycan lyases. Their disaccharides composition and fine structures were analyzed with SAX-HPLC. The results indicated that GAGs in rat kidneys were mainly composed of heparan sulfate and small amount of dermatan sulfate. Eight kinds of disaccharides were found in heparan sulfate, and the content of acetyl-containing disaccharides was 77.6%, while nonsulfated disaccharides were 59.7%. In dermatan sulfate, six kinds of disaccharides were found. The content of monosulfated disaccharides was 49.8%, and nonsulfated disaccharides were 32.9%.
通过两步酶解和离子交换色谱法从Wistar大鼠肾脏中提取并纯化糖胺聚糖（GAGs）。通过醋酸纤维素膜电泳鉴定GAGs的种类，并在弱阴离子交换柱上进行分离。那些纯化的GAGs用特定的糖胺聚糖裂解酶进一步完全水解。用SAX-HPLC分析其二糖组成和精细结构。结果表明，大鼠肾脏中的GAGs主要由硫酸乙酰肝素和少量硫酸皮肤素组成。在硫酸乙酰肝素中发现了八种二糖，含乙酰基二糖的含量为77.6%，而无硫酸化二糖为59.7%。在硫酸皮肤素中，发现了六种二糖。单硫酸化二糖的含量为49.8%，无硫酸化二糖为32.9%。
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