特立尼达森林媒介病毒的分离与鉴定：地方性传播和一种新的穆坎博病毒潜在媒介。
Isolation and characterization of sylvatic mosquito-borne viruses in Trinidad: enzootic transmission and a new potential vector of Mucambo virus.
机构信息
Department of Preclinical Sciences, and School of Veterinary Medicine, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Republic of Trinidad and Tobago.
出版信息
Am J Trop Med Hyg. 2010 Dec;83(6):1262-5. doi: 10.4269/ajtmh.2010.10-0280.
Mosquito surveillance was carried out in three forested regions of Trinidad during July 2007-March 2009. A total of 185,397 mosquitoes representing at least 46 species was collected, divided into pools of 1-50 mosquitoes according to species and sex, and screened for arboviruses using cytopathic effect assays on Vero cell monolayers. Eighty-five viruses were isolated, including members of the genera Alphavirus (Mucambo virus; MUCV) and Orthobunyavirus (Caraparu, Oriboca, Bimiti, and Wyeomyia viruses). Species of the Culex subgenus Melanoconion accounted for 56% of the total number of mosquitoes collected and 97% of the viruses isolated; Cx. (Mel.) portesi accounted for 92% of virus isolations. Our results also implicate for the first time Aedes (Ochlerotatus) hortator as a potential vector of MUCV. Phylogenetic analyses of 43 MUCV strains suggest population subdivision within Trinidad, consistent with the hypothesis of enzootic maintenance in localized rodent populations.
2007 年 7 月至 2009 年 3 月，在特立尼达的三个森林地区进行了蚊虫监测。共收集了 185397 只蚊子，至少代表 46 个物种，根据物种和性别将其分为 1-50 只蚊子的群集，并使用 Vero 细胞单层的细胞病变效应测定法对虫媒病毒进行筛查。共分离出 85 种病毒，包括属甲病毒（Mucambo 病毒；MUCV）和布尼亚病毒（Caraparu、Oriboca、Bimiti 和 Wyeomyia 病毒）的成员。属于库蚊亚属的 Melanoconion 的物种占收集的蚊子总数的 56%，占分离出的病毒总数的 97%；Cx.（Mel.）portesi 占病毒分离的 92%。我们的结果还首次表明，埃及伊蚊（Ochlerotatus）hortator 可能是 MUCV 的潜在传播媒介。43 株 MUCV 株的系统发育分析表明，特立尼达存在种群内部分区，与局部啮齿动物种群中地方性维持的假设一致。
Zotero 插件