[枯草芽孢杆菌中核黄素生物合成操纵子调控区域的异常结构]
[Unusual structure of the regulatory region of the riboflavin biosynthesis operon in Bacillus subtilis].
作者信息
Mironov V N, Perumov D A, Kraev A S, Stepanov A I, Skriabin K G
出版信息
Mol Biol (Mosk). 1990 Jan-Feb;24(1):256-61.
In vitro mutagenesis with methylhydroxylamine and nitrosomethylurea was used to obtain a number of Bacillus subtilis mutants impaired in flavin-dependent response. Mutants displayed varying degree of flavin-dependent repression of riboflavin synthase and of 6,7-dimethyl-8-ribityl-lumasine accumulation. Single nucleotide substitutions were detected by DNA sequencing in all of the mutants, affecting the 48 b.p. target area between the mRNA start and the AUG of the first gene.
利用甲基羟胺和亚硝基甲基脲进行体外诱变，以获得一些在黄素依赖性反应中受损的枯草芽孢杆菌突变体。这些突变体在核黄素合酶的黄素依赖性抑制以及6,7 - 二甲基 - 8 - 核糖基 - 鲁马辛积累方面表现出不同程度的变化。通过DNA测序在所有突变体中检测到单核苷酸替换，这些替换影响了第一个基因的mRNA起始位点与AUG之间的48个碱基对的目标区域。
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