一种用于汞（II）离子检测的新型单标记荧光寡核苷酸探针：利用脱氧鸟苷的固有淬灭作用。
A novel single-labeled fluorescent oligonucleotide probe for mercury(II) ion detection: using the inherent quenching of deoxyguanosines.
机构信息
State Key Lab of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China.
出版信息
J Fluoresc. 2011 May;21(3):1049-52. doi: 10.1007/s10895-010-0785-z. Epub 2010 Dec 9.
PMID:21152961
Abstract
A novel single-labeled fluorescent oligonucleotide (OND) probe for the detection of nanomolar mercury(II) ion in aqueous solution is developed based on the inherent quenching of deoxyguanosines. The formation of hairpin structure of OND-Hg(2+) complex brings deoxyguanosines close to dye, resulting in decreased dye fluorescence due to photoinduced electron transfer from dye to deoxyguanosines.
摘要
基于脱氧鸟苷固有猝灭作用，开发了一种用于检测水溶液中纳摩尔汞(II)离子的新型单标记荧光寡核苷酸（OND）探针。OND-Hg(2+) 复合物形成发夹结构，使脱氧鸟苷靠近染料，由于染料到脱氧鸟苷的光致电子转移，导致染料荧光降低。
Zotero 插件