[吗啡对大鼠海马神经元突触传递的影响及机制]

[Effects and mechanisms of morphine on synaptic transmission of hippocampal neurons of rat].

作者信息

Yue Wei-dong, Zhang Yun-hong, Li Yu-rong, Zhang Shu-zhuo, Yang Lei

机构信息

Department of Neurology, Harbin Medical University, Harbin 150086, China.

出版信息

Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2003 May;19(2):150-3.

PMID:21207663

Abstract

AIM

To investigate the effects of morphine on synaptic transmission of neurons of central nervous system and reveal the mechanism underlying it.

METHODS

New born wistar rats were used for primary culture of hippocampus neurons. Using whole-cell patch-clamp technique, we observed the excitatory and spontaneous inhibitory postsynaptic current (EPSC, sIPSC) and glutamate-induced current before and after morphine treatment.

RESULTS

(1) sEPSC of hippocampal neurons was markedly increased after morphine application. The effect of morphine was blocked by opioid antagonist naloxone (n=18, P < 0.01). (2) The frequency of mEPSC and the amplitude of glutamate-induced current of hippocampal neurons had no significant changes after morphine treatment (P > 0.05). (3) Morphine inhibited sIPSC of hippocampal neurons markedly and naloxone could block this effect (n=13, P < 0.01).

CONCLUSION

The results suggest that the exciting effect of morphine on hippocampal neurons are not due to direct influence of morphine on glutamate synapses transmission, but may result from the inhibition on interneurons, that is "disinhibition" way.

摘要

目的

研究吗啡对中枢神经系统神经元突触传递的影响并揭示其潜在机制。

方法

采用新生Wistar大鼠进行海马神经元原代培养。运用全细胞膜片钳技术，观察吗啡处理前后兴奋性和自发性抑制性突触后电流（EPSC、sIPSC）以及谷氨酸诱导电流。

结果

（1）应用吗啡后海马神经元的sEPSC显著增加。吗啡的作用被阿片类拮抗剂纳洛酮阻断（n = 18，P < 0.01）。（2）吗啡处理后海马神经元的微小兴奋性突触后电流（mEPSC）频率和谷氨酸诱导电流幅度无显著变化（P > 0.05）。（3）吗啡显著抑制海马神经元的sIPSC，纳洛酮可阻断此效应（n = 13，P < 0.01）。