CstF-50 同源二聚化结构域支持的 CstF 六聚体结构。
Hexameric architecture of CstF supported by CstF-50 homodimerization domain structure.
机构信息
Institut Européen de Chimie et Biologie, Institut National de la Santé et de la Recherche Médicale (INSERM) U869, Pessac, France.
出版信息
RNA. 2011 Mar;17(3):412-8. doi: 10.1261/rna.2481011. Epub 2011 Jan 13.
Abstract
The Cleavage stimulation Factor (CstF) complex is composed of three subunits and is essential for pre-mRNA 3'-end processing. CstF recognizes U and G/U-rich cis-acting RNA sequence elements and helps stabilize the Cleavage and Polyadenylation Specificity Factor (CPSF) at the polyadenylation site as required for productive RNA cleavage. Here, we describe the crystal structure of the N-terminal domain of Drosophila CstF-50 subunit. It forms a compact homodimer that exposes two geometrically opposite, identical, and conserved surfaces that may serve as binding platform. Together with previous data on the structure of CstF-77, homodimerization of CstF-50 N-terminal domain supports the model in which the functional state of CstF is a heterohexamer.
摘要
剪接刺激因子（CstF）复合物由三个亚基组成，是前体 mRNA 3'-端加工所必需的。CstF 识别 U 和 U/G 丰富的顺式作用 RNA 序列元件，并帮助稳定切割和多聚腺苷酸化特异性因子（CPSF）在多聚腺苷酸化位点，以进行有效的 RNA 切割。在这里，我们描述了果蝇 CstF-50 亚基的 N 端结构域的晶体结构。它形成一个紧凑的同源二聚体，暴露两个几何上相反的、相同的和保守的表面，可能作为结合平台。与 CstF-77 结构的先前数据一起，CstF-50 N 端结构域的同源二聚化支持 CstF 的功能状态是异六聚体的模型。
相似文献
1
Hexameric architecture of CstF supported by CstF-50 homodimerization domain structure.
RNA. 2011 Mar;17(3):412-8. doi: 10.1261/rna.2481011. Epub 2011 Jan 13.
2
Crystal structure of murine CstF-77: dimeric association and implications for polyadenylation of mRNA precursors.
Mol Cell. 2007 Mar 23;25(6):863-75. doi: 10.1016/j.molcel.2007.01.034.
3
Reconstitution of the CstF complex unveils a regulatory role for CstF-50 in recognition of 3'-end processing signals.
Nucleic Acids Res. 2018 Jan 25;46(2):493-503. doi: 10.1093/nar/gkx1177.
4
The structure of the CstF-77 homodimer provides insights into CstF assembly.
Nucleic Acids Res. 2007;35(13):4515-22. doi: 10.1093/nar/gkm458. Epub 2007 Jun 21.
5
The hinge domain of the cleavage stimulation factor protein CstF-64 is essential for CstF-77 interaction, nuclear localization, and polyadenylation.
J Biol Chem. 2010 Jan 1;285(1):695-704. doi: 10.1074/jbc.M109.061705. Epub 2009 Nov 3.
6
Fip1 is a multivalent interaction scaffold for processing factors in human mRNA 3' end biogenesis.
Elife. 2022 Sep 8;11:e80332. doi: 10.7554/eLife.80332.
7
Polyadenylation proteins CstF-64 and tauCstF-64 exhibit differential binding affinities for RNA polymers.
Biochem J. 2007 Feb 1;401(3):651-8. doi: 10.1042/BJ20061097.
8
Structural Insights into the Human Pre-mRNA 3'-End Processing Machinery.
Mol Cell. 2020 Feb 20;77(4):800-809.e6. doi: 10.1016/j.molcel.2019.11.005. Epub 2019 Dec 3.
9
Interactions of CstF-64, CstF-77, and symplekin: implications on localisation and function.
Mol Biol Cell. 2011 Jan 1;22(1):91-104. doi: 10.1091/mbc.E10-06-0543. Epub 2010 Nov 30.
10
Cytoplasmic CstF-77 protein belongs to a masking complex with cytoplasmic polyadenylation element-binding protein in Xenopus oocytes.
J Biol Chem. 2006 Sep 29;281(39):28687-98. doi: 10.1074/jbc.M601116200. Epub 2006 Aug 1.
引用本文的文献
1
Adaptation of the Protocol for the Isolation of Biotinylated Protein Complexes for Tissues.
Int J Mol Sci. 2025 Aug 19;26(16):8009. doi: 10.3390/ijms26168009.
2
CSTF3 contributes to platinum resistance in ovarian cancer through alternative polyadenylation of lncRNA NEAT1 and generating the short isoform NEAT1_1.
Cell Death Dis. 2024 Jun 19;15(6):432. doi: 10.1038/s41419-024-06816-1.
3
Molecular mechanism for the interaction between human CPSF30 and hFip1.
Genes Dev. 2020 Dec 1;34(23-24):1753-1761. doi: 10.1101/gad.343814.120. Epub 2020 Oct 29.
4
Recent molecular insights into canonical pre-mRNA 3'-end processing.
Transcription. 2020 Apr;11(2):83-96. doi: 10.1080/21541264.2020.1777047. Epub 2020 Jun 11.
5
Crystal structure of the LUFS domain of human single-stranded DNA binding Protein 2 (SSBP2).
Protein Sci. 2019 Apr;28(4):788-793. doi: 10.1002/pro.3581. Epub 2019 Feb 12.
6
The structural basis of CstF-77 modulation of cleavage and polyadenylation through stimulation of CstF-64 activity.
Nucleic Acids Res. 2018 Dec 14;46(22):12022-12039. doi: 10.1093/nar/gky862.
7
Targeting the Polyadenylation Signal of Pre-mRNA: A New Gene Silencing Approach for Facioscapulohumeral Dystrophy.
Int J Mol Sci. 2018 May 3;19(5):1347. doi: 10.3390/ijms19051347.
8
Reconstitution of the CstF complex unveils a regulatory role for CstF-50 in recognition of 3'-end processing signals.
Nucleic Acids Res. 2018 Jan 25;46(2):493-503. doi: 10.1093/nar/gkx1177.
9
Cleavage and polyadenylation: Ending the message expands gene regulation.
RNA Biol. 2017 Jul 3;14(7):865-890. doi: 10.1080/15476286.2017.1306171. Epub 2017 Apr 28.
10
The end of the message: multiple protein-RNA interactions define the mRNA polyadenylation site.
Genes Dev. 2015 May 1;29(9):889-97. doi: 10.1101/gad.261974.115.
本文引用的文献
1
[27] Maximum-likelihood heavy-atom parameter refinement for multiple isomorphous replacement and multiwavelength anomalous diffraction methods.
Methods Enzymol. 1997;276:472-494. doi: 10.1016/S0076-6879(97)76073-7.
2
Nuclear deadenylation/polyadenylation factors regulate 3' processing in response to DNA damage.
EMBO J. 2010 May 19;29(10):1674-87. doi: 10.1038/emboj.2010.59. Epub 2010 Apr 8.
3
A functional human Poly(A) site requires only a potent DSE and an A-rich upstream sequence.
EMBO J. 2010 May 5;29(9):1523-36. doi: 10.1038/emboj.2010.42. Epub 2010 Mar 25.
4
Molecular mechanisms of eukaryotic pre-mRNA 3' end processing regulation.
Nucleic Acids Res. 2010 May;38(9):2757-74. doi: 10.1093/nar/gkp1176. Epub 2009 Dec 30.
5
DANGLE: A Bayesian inferential method for predicting protein backbone dihedral angles and secondary structure.
J Magn Reson. 2010 Feb;202(2):223-33. doi: 10.1016/j.jmr.2009.11.008. Epub 2009 Dec 16.
6
Jalview Version 2--a multiple sequence alignment editor and analysis workbench.
Bioinformatics. 2009 May 1;25(9):1189-91. doi: 10.1093/bioinformatics/btp033. Epub 2009 Jan 16.
7
The BARD1 C-terminal domain structure and interactions with polyadenylation factor CstF-50.
Biochemistry. 2008 Nov 4;47(44):11446-56. doi: 10.1021/bi801115g. Epub 2008 Oct 9.
8
Conserved motifs in both CPSF73 and CPSF100 are required to assemble the active endonuclease for histone mRNA 3'-end maturation.
EMBO Rep. 2008 Oct;9(10):1013-8. doi: 10.1038/embor.2008.146. Epub 2008 Aug 8.
9
The structure of the CstF-77 homodimer provides insights into CstF assembly.
Nucleic Acids Res. 2007;35(13):4515-22. doi: 10.1093/nar/gkm458. Epub 2007 Jun 21.
10
Crystal structure of murine CstF-77: dimeric association and implications for polyadenylation of mRNA precursors.
Mol Cell. 2007 Mar 23;25(6):863-75. doi: 10.1016/j.molcel.2007.01.034.
Zotero 插件