斑点酶联免疫吸附测定法与夹心酶联免疫吸附测定法在检测班氏丝虫病患者循环抗原中的比较。

Comparison of Dot-ELISA with Sandwich ELISA in detecting circulating antigen in patients with bancroftian filariasis.

作者信息

Zheng H J, Fuhrman J A, Xu M, Cheng W F, Reddy M V, Piessens W F

机构信息

Department of Filariasis, Guizhou Provincial Institute of Parasitic Diseases, Guiyang.

出版信息

Chin Med J (Engl). 1990 Sep;103(9):709-12.

PMID:2123770

Abstract

Dot-ELISA assay was compared with standard Sandwich ELISA in detecting parasite antigen in sera from patients with bancroftian filariasis. The same monoclonal antibody and the same serum samples were used in both assays. With Dot-ELISA, 67 of 70 serum samples from microfilaremic patients were positive at a dilution of 1:50. End titers ranged from 1:80 to 1:1 280. While with Sandwich ELISA, 64 of the 70 serum samples were positive at a dilution of 1:10. End titers ranged from 1:10 to 1:320. The specificity of both assays was over 91%, but their sensitivity was markedly different. Dot-ELISA could detect as little as 0.055 ng/ml microfilarial antigen added to normal human serum, whereas the lower limit of detection by Sandwich-ELISA was 10 ng/ml parasite antigen. An additional advantage of Dot-ELISA is that it does not require radioactivity or sophisticated equipment and, therefore, can be performed in virtually all filariasis-endemic areas.

摘要

斑点酶联免疫吸附测定（Dot-ELISA）与标准夹心酶联免疫吸附测定（Sandwich ELISA）在检测班氏丝虫病患者血清中的寄生虫抗原方面进行了比较。两种测定均使用相同的单克隆抗体和相同的血清样本。采用Dot-ELISA法，70份来自微丝蚴血症患者的血清样本中有67份在1:50稀释度下呈阳性。最终滴度范围为1:80至1:1280。而采用夹心ELISA法时，70份血清样本中有64份在1:10稀释度下呈阳性。最终滴度范围为1:10至1:320。两种测定的特异性均超过91%，但它们的灵敏度明显不同。Dot-ELISA能够检测到添加到正常人血清中的低至0.055 ng/ml的微丝蚴抗原，而夹心ELISA的检测下限为10 ng/ml寄生虫抗原。Dot-ELISA的另一个优点是它不需要放射性物质或精密设备，因此几乎可以在所有丝虫病流行地区进行检测。