一种新的 HPTLC 密度测定法用于分析海芒果和商业制剂中的獐牙菜苦苷。
A new HPTLC densitometric method for analysis of swertiamarin in Enicostemma littorale and commercial formulations.
机构信息
Department of Pharmacognosy, College of Pharmacy, Al-Kharj University, Al-Kharj, Kingdom of Saudi Arabia.
出版信息
Nat Prod Res. 2011 Jan;25(1):17-25. doi: 10.1080/14786411003754348.
A high-performance thin layer chromatographic densitometric method for the analysis of swertiamarin in 60% methanolic extract of Enicostemma littorale and commercial formulations has been developed and validated in this study. Swertiamarin was separated on aluminium-backed silica gel 60 F₂₅₄ plates using ethyl acetate : methanol : water (77 : 15 : 8)% v/v as the mobile phase. This system was found to give a compact spot of swertiamarin at R(f) value 0.36 ± 0.01. The limit of detection and limit of quantification were found to be 31.25 and 103.12 ng spot⁻¹, respectively. The proposed method was employed with a high degree of precision and accuracy for the estimation of swertiamarin in methanolic extract of Enicostemma littorale and in commercial formulations.
本研究建立并验证了一种高效薄层色谱分光光度法，用于分析 60%甲醇提取物和市售制剂中环烯醚萜苷在网脉橐吾中的含量。环烯醚萜苷在铝-backed 硅胶 60 F₂₅₄ 板上，以乙酸乙酯：甲醇：水（77：15：8）% v/v 为流动相分离。该系统在 R(f) 值为 0.36 ± 0.01 时得到了一个紧凑的环烯醚萜苷斑点。检测限和定量限分别为 31.25 和 103.12 ng spot⁻¹。该方法用于甲醇提取物和市售制剂中环烯醚萜苷的测定，具有较高的精密度和准确性。
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