Wu Ying, Cheng Hui, Zou Hong-Da, Jia Dong-Mei, Zhao Chun-Yan, Yuan Ya-Ping
College of Plant Science, Jilin University, Changchun 130062, China.
Zhong Yao Cai. 2010 Sep;33(9):1363-5.
To clone Aralia elata squalene synthase gene (designated as AeSS) and construct plant expression vector for transgenic research.
Isolated squalene synthase from Aralia elata with specific primers by RT-PCR and inserted AeSS gene into the plant expression vector pBI121.
The full-length cDNA of AeSS (Genebank accession Number: GU354313) was 1 261 bp and contained a 1 245 bp open reading frame (ORF) encoding a polypeptide of 414 amino acids. The plant expression vector pAeSS was constructed by inserted AeSS gene into the downstream of 35 S promoter of plant expression vector pBI121.
AeSS gene was cloned and plant expression vector was constructed for future research.
克隆辽东楤木鲨烯合酶基因（命名为AeSS）并构建植物表达载体用于转基因研究。
通过RT-PCR用特异性引物从辽东楤木中分离鲨烯合酶，并将AeSS基因插入植物表达载体pBI121。
AeSS的全长cDNA（基因库登录号：GU354313）为1261 bp，包含一个1245 bp的开放阅读框（ORF），编码一个414个氨基酸的多肽。通过将AeSS基因插入植物表达载体pBI121的35S启动子下游构建了植物表达载体pAeSS。
克隆了AeSS基因并构建了植物表达载体，以供未来研究使用。
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