大麻二酚减轻脂多糖诱导的小鼠脑内血管改变和炎症：活体显微镜研究。
Cannabidiol reduces lipopolysaccharide-induced vascular changes and inflammation in the mouse brain: an intravital microscopy study.
机构信息
Laboratorio de Apoyo a la Investigación, Hospital Universitario Fundación Alcorcón and Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativa, Alcorcón, 28922, Madrid, Spain.
出版信息
J Neuroinflammation. 2011 Jan 18;8(1):5. doi: 10.1186/1742-2094-8-5.
BACKGROUND
The phytocannabinoid cannabidiol (CBD) exhibits antioxidant and antiinflammatory properties. The present study was designed to explore its effects in a mouse model of sepsis-related encephalitis by intravenous administration of lipopolysaccharide (LPS).
METHODS
Vascular responses of pial vessels were analyzed by intravital microscopy and inflammatory parameters measured by qRT-PCR.
RESULTS
CBD prevented LPS-induced arteriolar and venular vasodilation as well as leukocyte margination. In addition, CBD abolished LPS-induced increases in tumor necrosis factor-alpha and cyclooxygenase-2 expression as measured by quantitative real time PCR. The expression of the inducible-nitric oxide synthase was also reduced by CBD. Finally, preservation of Blood Brain Barrier integrity was also associated to the treatment with CBD.
CONCLUSIONS
These data highlight the antiinflammatory and vascular-stabilizing effects of CBD in endotoxic shock and suggest a possible beneficial effect of this natural cannabinoid.
背景
植物大麻素大麻二酚 (CBD) 具有抗氧化和抗炎特性。本研究旨在通过静脉注射脂多糖 (LPS) 来探索其在与脓毒症相关的脑炎的小鼠模型中的作用。
方法
通过活体显微镜分析脑膜血管的血管反应，并通过 qRT-PCR 测量炎症参数。
结果
CBD 可预防 LPS 诱导的小动脉和小静脉扩张以及白细胞边缘聚集。此外，CBD 还消除了 LPS 诱导的肿瘤坏死因子-α和环加氧酶-2 表达的增加，这是通过定量实时 PCR 测量的。诱导型一氧化氮合酶的表达也被 CBD 降低。最后，CBD 的治疗还与血脑屏障完整性的保存有关。
结论
这些数据强调了 CBD 在内毒素休克中的抗炎和血管稳定作用，并表明这种天然大麻素可能具有有益的作用。
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