环磷酸腺苷对红曲菌 M-7 生长和次级代谢产物的影响。

Effects of cyclic AMP on development and secondary metabolites of Monascus ruber M-7.

机构信息

College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei Province, China.

出版信息

Lett Appl Microbiol. 2011 Apr;52(4):420-6. doi: 10.1111/j.1472-765X.2011.03022.x. Epub 2011 Mar 1.

DOI:10.1111/j.1472-765X.2011.03022.x

PMID:21299575

Abstract

AIM

To study effects of cyclic adenosine monophosphate (cAMP) on development and secondary metabolites of Monascus ruber M-7.

METHODS AND RESULTS

Plate culture, liquid-state fermentation (LSF) and solid-state fermentation (SSF) were used to evaluate effects of cAMP on colonial growth, spore formation and polyketide production of Strain M-7. The results revealed that the variation trends of colonial sizes, numbers of sexual spores and red pigment contents of M-7 were in a dose-dependent manner. And generally they increased and decreased with cAMP concentrations in the ranges of low cAMP concentrations and high cAMP concentrations, respectively. But the variation trends of numbers of asexual spores and citrinin production in both LSF and SSF were opposite to those of colonial sizes, sexual sporulation and red pigment.

CONCLUSIONS

The regulation of cAMP on development and secondary metabolites in Strain M-7 was in a dose-dependent pattern. And red pigment might convert to citrinin under changing cAMP concentrations.

SIGNIFICANCE AND IMPACT OF THE STUDY

The effects of cAMP on Strain M-7 in SSF give a new clue to enhance beneficial polyketides and reduce citrinin produced by M. ruber.

摘要

目的

研究环磷酸腺苷（cAMP）对红曲霉 M-7 生长发育及次生代谢产物的影响。

方法与结果

采用平板培养、液体发酵（LSF）和固态发酵（SSF）的方法，考察 cAMP 对 M-7 菌株菌落生长、产孢及聚酮类物质合成的影响。结果表明，M-7 菌株的菌落大小、有性孢子数和红色素含量的变化趋势均呈剂量依赖性，分别在低浓度和高浓度 cAMP 范围内呈增加和减少的趋势。但 LSF 和 SSF 中无性孢子数和桔青霉素产量的变化趋势与菌落大小、有性孢子形成和红色素相反。